Skip to Main Content
Table II

Measurements of MukB and BsSMC Lengths and Calculations

Dimensions measured or calculatedMeasured lengthCalculated length of coil
      nm ± SD  nm 
1. Whole MukB, length  65.3 ± 2.6   
    n = 30   
2. MukB hinge, diam   6.7 ± 1.0   n = 52   
3. Outer globular domain, diam   7.3 ± 1.0   n = 17   
4. Coil length: hinge to outer  globular domain    51.3 
5. Whole MukBcoil, length  49.3 ± 2.6   n = 21   
6. Coil length: MukBcoil minus hinge    42.6 
7. Whole BsSMC, length  58.0 ± 1.1   n = 24   
8. Outer globular domain, diam   9.5 ± 0.9   n = 22   
9. BsSMC hinge, diam   7.1 ± 1.1   n = 23   
10. Coil length: hinge to globular domain    41.4 
Dimensions measured or calculatedMeasured lengthCalculated length of coil
      nm ± SD  nm 
1. Whole MukB, length  65.3 ± 2.6   
    n = 30   
2. MukB hinge, diam   6.7 ± 1.0   n = 52   
3. Outer globular domain, diam   7.3 ± 1.0   n = 17   
4. Coil length: hinge to outer  globular domain    51.3 
5. Whole MukBcoil, length  49.3 ± 2.6   n = 21   
6. Coil length: MukBcoil minus hinge    42.6 
7. Whole BsSMC, length  58.0 ± 1.1   n = 24   
8. Outer globular domain, diam   9.5 ± 0.9   n = 22   
9. BsSMC hinge, diam   7.1 ± 1.1   n = 23   
10. Coil length: hinge to globular domain    41.4 

All dimensions listed here have been corrected for the presumed 1-nm shell of metal. 1. The most definitive measurement was the length of the whole molecule in the folded-rod conformation and the lengths of single arms in the open-V; these were the same, and the measurements given here include both forms. 2. The smaller globular domain was identified as the hinge and had the same diameter in all three conformations. 3. Molecules in the open-V conformation usually showed the terminal domain resolved into two globular domains. The diameter of the terminal globular domain is given here. 4. The length of the coiled-coil was estimated by subtracting the diameters of the hinge and outer globular domains from the length of the whole molecule. 5. The length of the MukBcoil was measured from the outside of the globular hinge domain to the termination of the thin coil. This termination was sometimes ambiguous, hence the larger standard deviation of these measurements. 6. The length of the coil was estimated by subtracting the diameter of the hinge (2) from the length of MukBcoil (5). 7. The length of whole BsSMC molecules was measured, as in 1. 8. The larger (nonhinge) globular domain was approximately spherical in all conformations of BsSMC, and its diameter is measure here. 9. The diameter of the hinge domain, measured as in 2. 10. The coil of BsSMC was estimated by subtracting the diameters of the hinge and outer globular domain from the length of the whole molecule.  

Close Modal

or Create an Account

Close Modal
Close Modal