Yeast Strains and Plasmids Used in This Study
| Strain . | . | Genotype . | . | Source . |
|---|---|---|---|---|
| LM23-3az (parental) | MATabar1 [FUS1–lacZ::URA3] his4 leu2 met1 trp1 ura3–52 | Ref. 35 | ||
| SRM5 | MATaaxl1 (cef3) -1 bar1 [FUS1–lacZ::URA3] his4 leu2 met1 ura3–52 | This study | ||
| LE3-11B | MATaaxl1 (cef3) -1 bar1 leu2 his4 met1 trp1 | This study | ||
| LE1B3 | MATaaxl1 (cef3) -1 bar1 met1 ura3–52 | This study | ||
| LE6B3 | MATα axl1 (cef3) -1 bar1 met1 ura3–52 | This study | ||
| LM104 | Isogenic to LM23-3az but cured of [FUS1–lacZ::URA3] | This study | ||
| LE74x | Isogenic to LM104 but axl1 -Δ1 | This study | ||
| LEab1 | Isogenic to LM104 but rsr1 Δ::URA3 | This study | ||
| LEab3 | Isogenic to LM104 but bud3 Δ::URA3 | This study | ||
| LE281 | Isogenic to LM104 but fus1 -Δ1 | This study | ||
| EYL36 | MATahis4–34 trp1 -Δ1 ura3–52 fus2-Δ3 | Ref. 19 | ||
| LM110 | Isogenic to LM104 but Δste6::URA3 | Ref. 17 | ||
| IH1701 | MATα ade6 his2 | Ref. 17 | ||
| FC139 | MATα bar1 lys5 met1 ura3–52 | Ref. 17 | ||
| LE49a | Isogenic to FC139 but axl1 -Δ1 | This study | ||
| LE17a | Isogenic to FC139 but axl1 -Δ2 | This study | ||
| LEb1 | Isogenic to FC139 but rsr1 Δ::URA3 | This study | ||
| LEb3 | Isogenic to FC139 but bud3 Δ::URA3 | This study | ||
| LE49b1 | Isogenic to LE49a but axl1 -Δ1 rsr1 Δ::URA3 | This study | ||
| LE49b3 | Isogenic to LE49a but bud3 Δ::URA3 | This study | ||
| LEf14 | Isogenic to FC139 but fus1 Δ::URA3–1 | This study | ||
| LE272 | Isogenic to FC139 but fus2-Δ3 | This study | ||
| LE49f1-1 | Isogenic to LE49a but fus1Δ::URA3-1 | This study | ||
| LE49f2-1 | Isogenic to LE49a but fus2 Δ::URA3-1 | This study | ||
| RC757 | MATα sst2-1 his6 met1 can1 cyh2 rme | Ref. 6 | ||
| Plasmid | Description | |||
| p7-17e | YCp50 genomic library clone | This study | ||
| p7-17eΔ1 | Identical to p7-17e but axl1-Δ1 | This study | ||
| YCp17el | SalI 5.2-kb fragment containing an AXL1 allele in YCp50 | This study | ||
| pLEA7 | YIp5 containing axl1-Δ1 allele | This study | ||
| pPB181 | rsr1Δ::URA3 | Ref. 3 | ||
| pBUD3Δ#2 | bud3Δ::URA3 | Ref. 9 | ||
| pL32-GFP | RPL32 fused to GFP in pRS316 | J. Warner | ||
| pΔBA1 | axl1-Δ (848–1,142) allele in YCp50 | This study | ||
| p129 | AXL1 allele in pRS316 | Ref. 1 | ||
| p126 | axl1-H68A allele in pRS316 | Ref. 1 | ||
| p138 | axl1-E71A allele in pRS316 | Ref. 1 | ||
| YEp (RSR1) | RSR1 LEU2-2-μm based | Ref. 2 | ||
| YEp (rsr1val12) | rsr1val12 LEU2-2-μm based | Ref. 2 | ||
| pLE131 | ste6 (cef1-1) in YEp13 | Ref. 17 |
| Strain . | . | Genotype . | . | Source . |
|---|---|---|---|---|
| LM23-3az (parental) | MATabar1 [FUS1–lacZ::URA3] his4 leu2 met1 trp1 ura3–52 | Ref. 35 | ||
| SRM5 | MATaaxl1 (cef3) -1 bar1 [FUS1–lacZ::URA3] his4 leu2 met1 ura3–52 | This study | ||
| LE3-11B | MATaaxl1 (cef3) -1 bar1 leu2 his4 met1 trp1 | This study | ||
| LE1B3 | MATaaxl1 (cef3) -1 bar1 met1 ura3–52 | This study | ||
| LE6B3 | MATα axl1 (cef3) -1 bar1 met1 ura3–52 | This study | ||
| LM104 | Isogenic to LM23-3az but cured of [FUS1–lacZ::URA3] | This study | ||
| LE74x | Isogenic to LM104 but axl1 -Δ1 | This study | ||
| LEab1 | Isogenic to LM104 but rsr1 Δ::URA3 | This study | ||
| LEab3 | Isogenic to LM104 but bud3 Δ::URA3 | This study | ||
| LE281 | Isogenic to LM104 but fus1 -Δ1 | This study | ||
| EYL36 | MATahis4–34 trp1 -Δ1 ura3–52 fus2-Δ3 | Ref. 19 | ||
| LM110 | Isogenic to LM104 but Δste6::URA3 | Ref. 17 | ||
| IH1701 | MATα ade6 his2 | Ref. 17 | ||
| FC139 | MATα bar1 lys5 met1 ura3–52 | Ref. 17 | ||
| LE49a | Isogenic to FC139 but axl1 -Δ1 | This study | ||
| LE17a | Isogenic to FC139 but axl1 -Δ2 | This study | ||
| LEb1 | Isogenic to FC139 but rsr1 Δ::URA3 | This study | ||
| LEb3 | Isogenic to FC139 but bud3 Δ::URA3 | This study | ||
| LE49b1 | Isogenic to LE49a but axl1 -Δ1 rsr1 Δ::URA3 | This study | ||
| LE49b3 | Isogenic to LE49a but bud3 Δ::URA3 | This study | ||
| LEf14 | Isogenic to FC139 but fus1 Δ::URA3–1 | This study | ||
| LE272 | Isogenic to FC139 but fus2-Δ3 | This study | ||
| LE49f1-1 | Isogenic to LE49a but fus1Δ::URA3-1 | This study | ||
| LE49f2-1 | Isogenic to LE49a but fus2 Δ::URA3-1 | This study | ||
| RC757 | MATα sst2-1 his6 met1 can1 cyh2 rme | Ref. 6 | ||
| Plasmid | Description | |||
| p7-17e | YCp50 genomic library clone | This study | ||
| p7-17eΔ1 | Identical to p7-17e but axl1-Δ1 | This study | ||
| YCp17el | SalI 5.2-kb fragment containing an AXL1 allele in YCp50 | This study | ||
| pLEA7 | YIp5 containing axl1-Δ1 allele | This study | ||
| pPB181 | rsr1Δ::URA3 | Ref. 3 | ||
| pBUD3Δ#2 | bud3Δ::URA3 | Ref. 9 | ||
| pL32-GFP | RPL32 fused to GFP in pRS316 | J. Warner | ||
| pΔBA1 | axl1-Δ (848–1,142) allele in YCp50 | This study | ||
| p129 | AXL1 allele in pRS316 | Ref. 1 | ||
| p126 | axl1-H68A allele in pRS316 | Ref. 1 | ||
| p138 | axl1-E71A allele in pRS316 | Ref. 1 | ||
| YEp (RSR1) | RSR1 LEU2-2-μm based | Ref. 2 | ||
| YEp (rsr1val12) | rsr1val12 LEU2-2-μm based | Ref. 2 | ||
| pLE131 | ste6 (cef1-1) in YEp13 | Ref. 17 |