Role of Bud Site Selection Genes in Mating
| . | . | Relevant genotype . | . | Prezygote accumulation* . | . | Mating frequency‡ . | . | Bud site selection§ (axial) . |
|---|---|---|---|---|---|---|---|---|
| MATα strains | % | % | % | |||||
| FC139 | Parental | 5 | 21.0 ± 5.0 | 92 | ||||
| LE49a | axl1Δ | 55 | 6.0 ± 2.0 | 37 | ||||
| LEb1 | rsr1Δ | 60 | 4.0 ± 2.0 | 14 | ||||
| LEb3 | bud3Δ | 44 | 8.0 ± 0.6 | 29 | ||||
| LE49b1 | axl1Δ rsr1Δ | 64 | 5.0 ± 0.6 | 13 | ||||
| LE49b3 | axl1Δ bud3Δ | 64 | 5.0 ± 0.1 | 16 | ||||
| LEf14 | fus1Δ | 41 | 14.0 ± 0.9 | — | ||||
| MATa strains‖ | ||||||||
| LE74x | axl1Δ | 51 | 0.3 ± 0.1 | 40 | ||||
| LEab1 | rsr1Δ | 83 | 3.2 ± 0.4 | 17 | ||||
| LEab3 | bud3Δ | 64 | 2.6 ± 0.9 | — |
| . | . | Relevant genotype . | . | Prezygote accumulation* . | . | Mating frequency‡ . | . | Bud site selection§ (axial) . |
|---|---|---|---|---|---|---|---|---|
| MATα strains | % | % | % | |||||
| FC139 | Parental | 5 | 21.0 ± 5.0 | 92 | ||||
| LE49a | axl1Δ | 55 | 6.0 ± 2.0 | 37 | ||||
| LEb1 | rsr1Δ | 60 | 4.0 ± 2.0 | 14 | ||||
| LEb3 | bud3Δ | 44 | 8.0 ± 0.6 | 29 | ||||
| LE49b1 | axl1Δ rsr1Δ | 64 | 5.0 ± 0.6 | 13 | ||||
| LE49b3 | axl1Δ bud3Δ | 64 | 5.0 ± 0.1 | 16 | ||||
| LEf14 | fus1Δ | 41 | 14.0 ± 0.9 | — | ||||
| MATa strains‖ | ||||||||
| LE74x | axl1Δ | 51 | 0.3 ± 0.1 | 40 | ||||
| LEab1 | rsr1Δ | 83 | 3.2 ± 0.4 | 17 | ||||
| LEab3 | bud3Δ | 64 | 2.6 ± 0.9 | — |
Prezygote accumulation was determined by microscopic observation as described in Materials and Methods. More than 500 mating pairs were scored for each strain pair. MATa partner is LM23-3az.
Mating frequency was measured as the percent of prototrophic diploids formed/total viable cells after limited mating to the MATa partner, LM23-3az, and is the average ± standard deviation of duplicate filter mating determinations.
The frequency of axial budding (not bipolar or random) was determined. Budding patterns were assayed for >100 microcolonies.
Prezygote accumulation and mating frequenices determined as above. The MATα partner for both assays was FC139.