Double Mutant Analysis of AXL1, FUS1, and FUS2
| MATα strain . | . | Relevant genotype . | . | Prezygote accumulation* . | . | Mating frequency‡ . |
|---|---|---|---|---|---|---|
| % | % | |||||
| FC139 | AXL1 | 7 | 21.2 ± 6.3 | |||
| LEf14 | fus1Δ | 39 | 15.9 ± 0.0 | |||
| LE272 | fus2Δ | 38 | 14.8 ± 1.1 | |||
| LE49a | axl1Δ | 53 | 8.0 ± 0.1 | |||
| LE49f1-1 | axl1Δfus1Δ | 87 | 2.4 ± 0.7 | |||
| LE49f2-1 | axl1Δfus2Δ | 46 | 8.4 ± 0.1 |
| MATα strain . | . | Relevant genotype . | . | Prezygote accumulation* . | . | Mating frequency‡ . |
|---|---|---|---|---|---|---|
| % | % | |||||
| FC139 | AXL1 | 7 | 21.2 ± 6.3 | |||
| LEf14 | fus1Δ | 39 | 15.9 ± 0.0 | |||
| LE272 | fus2Δ | 38 | 14.8 ± 1.1 | |||
| LE49a | axl1Δ | 53 | 8.0 ± 0.1 | |||
| LE49f1-1 | axl1Δfus1Δ | 87 | 2.4 ± 0.7 | |||
| LE49f2-1 | axl1Δfus2Δ | 46 | 8.4 ± 0.1 |
Prezygote accumulation was determined as described in Materials and Methods. More than 200 mating pairs were scored for each strain pair. MATa partner was LM23-3az.
Mating frequency was measured as the percent of prototrophic diploids formed/total viable cells after limited mating to MATa partner, LM24-3az. Averages ± SD of duplicate filter mating determinations.