Quantitation of Nuclear Positioning by DAPI Staining
| Mating pair* . | . | Relevant genotype . | . | Nuclei aligned‡ . |
|---|---|---|---|---|
| % | ||||
| LE281/FCf14 | fus1Δ/fus1Δ | 97 | ||
| EYL36/FC272 | fus2Δ/fus2Δ | 16 | ||
| LE74x/LE17a | axl1Δ/axl1Δ | 8 | ||
| LEab1/LEb1 | rsr1Δ/rsr1Δ | 22 |
| Mating pair* . | . | Relevant genotype . | . | Nuclei aligned‡ . |
|---|---|---|---|---|
| % | ||||
| LE281/FCf14 | fus1Δ/fus1Δ | 97 | ||
| EYL36/FC272 | fus2Δ/fus2Δ | 16 | ||
| LE74x/LE17a | axl1Δ/axl1Δ | 8 | ||
| LEab1/LEb1 | rsr1Δ/rsr1Δ | 22 |
MATa and MATα cells were mated on filters for 3.5 h at 30°C (refer to Materials and Methods). Mating pairs were: LE281 (MATa fus1-Δ1)/FCf14 (MATα fus1Δ::URA3), EYL36 (MATa fus2-Δ3)/LE272 (MATα fus2-Δ1), LE74x (MATa axl1-Δ1)/LE17a (MATα axl1-Δ2), and LEab1 (MATa rsr1Δ::URA3)/LEb1 (MATα rsr1Δ::URA3).
Mating mixtures were stained with DAPI to visualize nuclei (refer to Materials and Methods). More than 30 mating pairs were scored for each strain combination.