Role of AXL1 Protease Functions in Cell Fusion
| Plasmid . | . | Allele . | . | a-Factor production* . | . | Prezygote accumulation‡ . | . | Bud site selection§ (axial) . |
|---|---|---|---|---|---|---|---|---|
| % | % | |||||||
| YCp17e1 | pAXL1 | + | 8 | 96 | ||||
| YCp50 | Vector | − | 60 | 42 | ||||
| p126 | pAxl1-H68A | − | 76 | 91 | ||||
| p138 | pAxl1-E71A | − | 60 | 99 | ||||
| pΔBA1 | pAxl1-Δ (848–1,142) | − | 61 | 92 |
| Plasmid . | . | Allele . | . | a-Factor production* . | . | Prezygote accumulation‡ . | . | Bud site selection§ (axial) . |
|---|---|---|---|---|---|---|---|---|
| % | % | |||||||
| YCp17e1 | pAXL1 | + | 8 | 96 | ||||
| YCp50 | Vector | − | 60 | 42 | ||||
| p126 | pAxl1-H68A | − | 76 | 91 | ||||
| p138 | pAxl1-E71A | − | 60 | 99 | ||||
| pΔBA1 | pAxl1-Δ (848–1,142) | − | 61 | 92 |
a-Factor production determined by halo assay (1, 17, 38) with plasmids in a MATα axl1 strain (LE1B).
Plasmids were assayed in the MATα axl1-Δ1 strain, LE49a, mated to LM23-3az. More than 200 mating pairs were counted per cross.
The frequency of axial (neither bipolar nor random) budding was determined. Budding patterns were assayed for >100 microcolonies.