Rockefeller University Press

Table VII

Ability of the Activated Allele of RSR1, rsr1^val12, to Function in Cell Fusion and Bud Site Selection

MATa strain/plasmid	RSR1 allele	Prezygote accumulation^*	Bud site selection^‡
			Axial	Bipolar	Random
		%	%	%	%
LEab1/pPB161	wt	7.2	84.0	7.0	9.0
LEab1/pPB264	rsr1^val12	51.4	15.6	12.8	71.6
LM104/pPB161	wt, wt	3.8	88.3	9.8	1.9
LM104/pPB264	wt, rsr1^val12	41.0	24.4	46.5	29.1

MATa strain/plasmid	RSR1 allele	Prezygote accumulation^*	Bud site selection^‡
			Axial	Bipolar	Random
		%	%	%	%
LEab1/pPB161	wt	7.2	84.0	7.0	9.0
LEab1/pPB264	rsr1^val12	51.4	15.6	12.8	71.6
LM104/pPB161	wt, wt	3.8	88.3	9.8	1.9
LM104/pPB264	wt, rsr1^val12	41.0	24.4	46.5	29.1

Prezygote accumulation was determined by microscopic observation as described in Materials and Methods. More than 200 mating pairs were scored for each strain pair. The MATα partner was FC139.

‡

Budding patterns were observed for >100 microcolonies.

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