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Table III

Genetic Interactions between bni1Δ and Motor Mutations

23°C37°C14°C30°C
Growth phenotype of         
 single mutants         
  bni1Δ  +++  ++  +++  ND 
  kip3Δ  +++  +++  +++  ND 
  dyn1Δ  +++  +++  +++  ND 
  kar3Δ   −   
  kip1Δ  +++  +++  +++  ND 
  kip2Δ  +++  +++  +++  ND 
  cin8Δ  +++  +/−  +++  ND 
  smy1Δ  +++  +++  +++  ND 
Growth phenotype of         
 double mutants         
  bni1Δ kip3Δ  +++  ++  +++  ND 
  bni1Δ dyn1Δ  ++  −  −  ND 
  bni1Δ kar3Δ   −   − 
  bni1Δ kip1Δ  +++  ++  +++  ND 
  bni1Δ kip2Δ  +++  ++  +++  ND 
  bni1Δ cin8Δ  +++  −  +++  ND 
  bni1Δ smy1Δ  +++  ++  +++  ND 
23°C37°C14°C30°C
Growth phenotype of         
 single mutants         
  bni1Δ  +++  ++  +++  ND 
  kip3Δ  +++  +++  +++  ND 
  dyn1Δ  +++  +++  +++  ND 
  kar3Δ   −   
  kip1Δ  +++  +++  +++  ND 
  kip2Δ  +++  +++  +++  ND 
  cin8Δ  +++  +/−  +++  ND 
  smy1Δ  +++  +++  +++  ND 
Growth phenotype of         
 double mutants         
  bni1Δ kip3Δ  +++  ++  +++  ND 
  bni1Δ dyn1Δ  ++  −  −  ND 
  bni1Δ kar3Δ   −   − 
  bni1Δ kip1Δ  +++  ++  +++  ND 
  bni1Δ kip2Δ  +++  ++  +++  ND 
  bni1Δ cin8Δ  +++  −  +++  ND 
  bni1Δ smy1Δ  +++  ++  +++  ND 

Each strain was struck on a YPD plate and incubated at the appropriate temperatures. For 23°, 30°, and 37°C test, plates were scored after 48 h. For 14°C test, plates were scored after 7 d.  

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