Mating Projection Formation, Quantitative Mating Assay, and Bipolar Bud Site Selection
| . | . | Mating projection formation . | . | Mating efficiency . | . | Bipolar bud site selection . |
|---|---|---|---|---|---|---|
| % | % | % | ||||
| bni1::kanr {BNI1} | 83.4 ± 5.5 | 0.17 ± 0.041 | — | |||
| bni1::kanr {bni1-CTΔ1} | 77.2 ± 5.2 | 0.095 ± 0.013 | — | |||
| bni1::kanr {CEN URA3} | 0 | 0.001 ± 0.0006 | — | |||
| BNI1/BNI1 | — | — | 4 ± 1.8 | |||
| bni1Δ/bni1Δ {BNI1} | — | — | 31 ± 2.3 | |||
| bni1Δ/bni1Δ {bni1-CTΔ1} | — | — | 92 ± 3.5 | |||
| bni1Δ/bni1Δ {CEN URA3} | — | — | 93 ± 2.1 |
| . | . | Mating projection formation . | . | Mating efficiency . | . | Bipolar bud site selection . |
|---|---|---|---|---|---|---|
| % | % | % | ||||
| bni1::kanr {BNI1} | 83.4 ± 5.5 | 0.17 ± 0.041 | — | |||
| bni1::kanr {bni1-CTΔ1} | 77.2 ± 5.2 | 0.095 ± 0.013 | — | |||
| bni1::kanr {CEN URA3} | 0 | 0.001 ± 0.0006 | — | |||
| BNI1/BNI1 | — | — | 4 ± 1.8 | |||
| bni1Δ/bni1Δ {BNI1} | — | — | 31 ± 2.3 | |||
| bni1Δ/bni1Δ {bni1-CTΔ1} | — | — | 92 ± 3.5 | |||
| bni1Δ/bni1Δ {CEN URA3} | — | — | 93 ± 2.1 |
The mating projection assay measured the percentage of cells with mating projections after 3 h in α factor (n = 300). The quantitative mating assay (Sprague, 1991) measured the percentage of diploids formed after a 4-h mating with the far1-c tester strain. The result of the bipolar bud site selection assay (Pringle et al., 1989) shows the percentage of cells with a random budding pattern.