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Table V

Mating Projection Formation, Quantitative Mating Assay, and Bipolar Bud Site Selection

Mating projection formationMating efficiencyBipolar bud site selection
  %  %  % 
bni1::kanr {BNI1}  83.4 ± 5.5   0.17 ± 0.041  — 
bni1::kanr {bni1-CTΔ1}  77.2 ± 5.2  0.095 ± 0.013  — 
bni1::kanr {CEN URA3}   0  0.001 ± 0.0006  — 
BNI1/BNI1  —  —   4 ± 1.8 
bni1Δ/bni1Δ {BNI1}  —  —  31 ± 2.3 
bni1Δ/bni1Δ {bni1-CTΔ1}  —  —  92 ± 3.5 
bni1Δ/bni1Δ {CEN URA3}  —  —  93 ± 2.1 
Mating projection formationMating efficiencyBipolar bud site selection
  %  %  % 
bni1::kanr {BNI1}  83.4 ± 5.5   0.17 ± 0.041  — 
bni1::kanr {bni1-CTΔ1}  77.2 ± 5.2  0.095 ± 0.013  — 
bni1::kanr {CEN URA3}   0  0.001 ± 0.0006  — 
BNI1/BNI1  —  —   4 ± 1.8 
bni1Δ/bni1Δ {BNI1}  —  —  31 ± 2.3 
bni1Δ/bni1Δ {bni1-CTΔ1}  —  —  92 ± 3.5 
bni1Δ/bni1Δ {CEN URA3}  —  —  93 ± 2.1 

The mating projection assay measured the percentage of cells with mating projections after 3 h in α factor (n = 300). The quantitative mating assay (Sprague, 1991) measured the percentage of diploids formed after a 4-h mating with the far1-c tester strain. The result of the bipolar bud site selection assay (Pringle et al., 1989) shows the percentage of cells with a random budding pattern.  

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