Morphometric Analysis of Presynaptic Terminals of Cultured Hippocampal Neurons before and after Hypertonic Stimulation
| . | . | Numbers of Synaptic Vesicles per Unit Area in Presynaptic Terminals . | ||||||
|---|---|---|---|---|---|---|---|---|
| Wild-type . | . | Synapsin I−/ . | ||||||
| Excitatory . | . | Inhibitory . | Excitory . | . | Inhibitory . | |||
| Control | 233 ± 15 (n = 10) | 209 ± 60 (n = 14) | 225 ± 31 (n = 12) | 245 ± 47 (n = 10) | ||||
| Stimulated | 202 ± 29 (n = 12) | 176 ± 27 (n = 12) | 209 ± 20 (n = 8) | 139 ± 25* (n = 10) | ||||
| Numbers of Synaptic Vesicles per Unit Area within 200 nm from the Synaptic Cleft | ||||||||
| Wild-type | Synapsin I−/ | |||||||
| Excitatory | Inhibitory | Excitatory | Inhibitory | |||||
| Control | 341 ± 66 (n = 10) | 270 ± 76 (n = 14) | 310 ± 46 (n = 12) | 335 ± 28 (n = 10) | ||||
| Stimulated | 283 ± 45 (n = 12) | 259 ± 30 (n = 12) | 269 ± 50 (n = 8) | 304 ± 72 (n = 10) | ||||
| . | . | Numbers of Synaptic Vesicles per Unit Area in Presynaptic Terminals . | ||||||
|---|---|---|---|---|---|---|---|---|
| Wild-type . | . | Synapsin I−/ . | ||||||
| Excitatory . | . | Inhibitory . | Excitory . | . | Inhibitory . | |||
| Control | 233 ± 15 (n = 10) | 209 ± 60 (n = 14) | 225 ± 31 (n = 12) | 245 ± 47 (n = 10) | ||||
| Stimulated | 202 ± 29 (n = 12) | 176 ± 27 (n = 12) | 209 ± 20 (n = 8) | 139 ± 25* (n = 10) | ||||
| Numbers of Synaptic Vesicles per Unit Area within 200 nm from the Synaptic Cleft | ||||||||
| Wild-type | Synapsin I−/ | |||||||
| Excitatory | Inhibitory | Excitatory | Inhibitory | |||||
| Control | 341 ± 66 (n = 10) | 270 ± 76 (n = 14) | 310 ± 46 (n = 12) | 335 ± 28 (n = 10) | ||||
| Stimulated | 283 ± 45 (n = 12) | 259 ± 30 (n = 12) | 269 ± 50 (n = 8) | 304 ± 72 (n = 10) | ||||
Different from the value for wild-type at P < 0.05. The mean ± SD of the number of synaptic vesicles/μm2 (n represents the number of synapses examined) is shown. t test was used to determine the significance of the changes.