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Table 1

Analysis of the Tryptic Peptide Profiles of 11 Major Exosomal Proteins by MALDI-TOF-MS

BandTotal no. of peptidesProtein identified (NCBI accession no.)Matching peptidesEstimated protein molecular massPercentage of the protein covered
    kD 
15 Mac-1 α chain (X07640) 11 127.5 12 
20 n.i.    
19 mP47 = MFG-E8 (Y11684) 11 45 24 
28 hsc73 (U73744) + 11 70.9 20 
  mP47 = MFG-E8 (Y11684) 10 45 23 
15 mP47 = MFG-E8 (Y11684) 12 45 29 
actin (M12481) 41.7 20 
11 G protein Gi2α subunit (P08752) 40.1 32 
calpactin I heavy chain = annexin II (M14044) 38.7 24 
MRV provirus gag (core shell protein p30) (S80082) + 60.4 16 
  MHC II β chain (P14483) 30.1 22 
10 CD9 (P40240) + nonidentified 25.2 11 
11 10 nonidentified    
BandTotal no. of peptidesProtein identified (NCBI accession no.)Matching peptidesEstimated protein molecular massPercentage of the protein covered
    kD 
15 Mac-1 α chain (X07640) 11 127.5 12 
20 n.i.    
19 mP47 = MFG-E8 (Y11684) 11 45 24 
28 hsc73 (U73744) + 11 70.9 20 
  mP47 = MFG-E8 (Y11684) 10 45 23 
15 mP47 = MFG-E8 (Y11684) 12 45 29 
actin (M12481) 41.7 20 
11 G protein Gi2α subunit (P08752) 40.1 32 
calpactin I heavy chain = annexin II (M14044) 38.7 24 
MRV provirus gag (core shell protein p30) (S80082) + 60.4 16 
  MHC II β chain (P14483) 30.1 22 
10 CD9 (P40240) + nonidentified 25.2 11 
11 10 nonidentified    
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