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Table 2

Distribution of SPP in Membrane and Cytosol Fractions

SPPSPP
FractionVectorSPHKVectorSPHK
 pmol/mg protein pmol/nmol phospholipid 
Cytosol 0.45 ± 0.014 2.17 ± 0.012 0.015 ± 0.003 0.111 ± 0.044 
Membrane 1.7 ± 0.16 9.1 ± 0.37 0.009 ± 0.003 0.056 ± 0.007 
SPPSPP
FractionVectorSPHKVectorSPHK
 pmol/mg protein pmol/nmol phospholipid 
Cytosol 0.45 ± 0.014 2.17 ± 0.012 0.015 ± 0.003 0.111 ± 0.044 
Membrane 1.7 ± 0.16 9.1 ± 0.37 0.009 ± 0.003 0.056 ± 0.007 

SPP in cytosol and particulate fractions of HEK 293 cells transfected with c-myc–pcDNA3 (Vector) or c-myc–pcDNA3-SPHK1a (SPHK) was measured as described in Materials and Methods. The same samples were also analyzed for phospholipid content.

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