Relative Protein Levels of Peripherin Compared with NF-L in Cytoskeletal Fractions of Dorsal and Ventral Roots
| Tissues . | Genotype . | Ratio of peripherin to NF-L . |
|---|---|---|
| Ventral root | WT (n = 5) | 0.090 ± 0.037 |
| TPer (n = 3) | 0.650 ± 0.092 | |
| Per (n = 4) | 0.870 ± 0.130 | |
| Dorsal root | WT (n = 5) | 0.316 ± 0.164 |
| TPer (n = 3) | 0.590 ± 0.095 | |
| Per (n = 4) | 1.180 ± 0.057 |
| Tissues . | Genotype . | Ratio of peripherin to NF-L . |
|---|---|---|
| Ventral root | WT (n = 5) | 0.090 ± 0.037 |
| TPer (n = 3) | 0.650 ± 0.092 | |
| Per (n = 4) | 0.870 ± 0.130 | |
| Dorsal root | WT (n = 5) | 0.316 ± 0.164 |
| TPer (n = 3) | 0.590 ± 0.095 | |
| Per (n = 4) | 1.180 ± 0.057 |
20 μm of Triton X-100–insoluble fractions were fractionated on SDS-PAGE and stained with Coomassie blue. The OD corresponding to the peripherin and NF-L bands were then evaluated by densitometry. Ratios were calculated by dividing the peripherin OD by the NF-L OD for each mice. Results are mean ± SD.