F-actin Quantitation in Cells Microinjected with Anticofilin Antibodies
| . | Control IgG . | Ab286 . |
|---|---|---|
| Number of experiments | 4 | 6 |
| Total noninjected cells | 347 | 536 |
| Total injected cells | 260 | 385 |
| Ratio cell area injected/noninjected | 0.97 ± 0.03 | 1.02 ± 0.04 |
| Ratio mean intensity of F-actin | 1.09 ± 0.01 | 1.12 ± 0.02 |
| injected/noninjected |
| . | Control IgG . | Ab286 . |
|---|---|---|
| Number of experiments | 4 | 6 |
| Total noninjected cells | 347 | 536 |
| Total injected cells | 260 | 385 |
| Ratio cell area injected/noninjected | 0.97 ± 0.03 | 1.02 ± 0.04 |
| Ratio mean intensity of F-actin | 1.09 ± 0.01 | 1.12 ± 0.02 |
| injected/noninjected |
Area and F-actin content were quantified using NIH Image on images as described in Materials and Methods. Both control nonimmune rabbit IgG and Ab286 anticofilin were microinjected at a concentration of 6 mg/ml. 1–2 h after microinjection, the cells were stimulated for 1 min with EGF and fixed and stained for F-actin.