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Table 2

Brown Fat Microsomal Fatty Acyl Chain Elongation Activity in Warm- and Cold-acclimated Mice

SubstrateBAT (28°C)BAT (4°C)Relative increase in coldP value
 pmol Mal-CoA/min/mg prot ×  
palmitoyl CoA (C161.80 ± 0.64 8.50 ± 1.51 4.7 0.004 
arachidoyl CoA (C200.37 ± 0.05 1.57 ± 0.52 4.3 0.065 
lignoceroyl CoA (C240.33 ± 0.06 1.12 ± 0.56 3.4 0.229 
SubstrateBAT (28°C)BAT (4°C)Relative increase in coldP value
 pmol Mal-CoA/min/mg prot ×  
palmitoyl CoA (C161.80 ± 0.64 8.50 ± 1.51 4.7 0.004 
arachidoyl CoA (C200.37 ± 0.05 1.57 ± 0.52 4.3 0.065 
lignoceroyl CoA (C240.33 ± 0.06 1.12 ± 0.56 3.4 0.229 

Total fatty acid chain elongation activities in the presence of NADPH were determined in microsomal fractions prepared from brown adipose tissue (BAT) of mice either kept at thermoneutral temperature (28°C) or exposed to the cold for 3 d (4°C). Specific activity was expressed as picomoles of radioactive malonyl CoA (Mal-CoA) incorporated into hydrophobic long chain fatty acid fraction by 1 mg of microsomal protein in 1 min. Statistical significance of the increase was determined by unpaired t test.

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