Phospholipid Compositions of CHO-K1 and R-41 Mutant Cells Cultured in Ethanolamine-deficient Medium
| . | Percentage of total phospholipids . | |||||
|---|---|---|---|---|---|---|
| Strain . | PE . | PS . | PC . | SM . | PI . | Others . |
| CHO-K1 | 12.6 ± 1.3 | 5.4 ± 0.6 | 61.2 ± 5.2 | 12.1 ± 0.8 | 5.1 ± 0.6 | 3.5 ± 0.4 |
| R-41 | 5.6 ± 0.8 | 4.6 ± 0.4 | 70.4 ± 6.1 | 11.8 ± 1.2 | 5.5 ± 0.8 | 2.1 ± 0.6 |
| R-41 + PE | 14.7 ± 1.3 | 5.7 ± 0.6 | 60.4 ± 4.2 | 10.3 ± 1.2 | 5.7 ± 0.9 | 2.7 ± 0.2 |
| R-41 + Etn | 15.8 ± 0.9 | 5.5 ± 0.4 | 59.7 ± 3.9 | 10.1 ± 0.9 | 5.9 ± 1.1 | 3.1 ± 0.3 |
| . | Percentage of total phospholipids . | |||||
|---|---|---|---|---|---|---|
| Strain . | PE . | PS . | PC . | SM . | PI . | Others . |
| CHO-K1 | 12.6 ± 1.3 | 5.4 ± 0.6 | 61.2 ± 5.2 | 12.1 ± 0.8 | 5.1 ± 0.6 | 3.5 ± 0.4 |
| R-41 | 5.6 ± 0.8 | 4.6 ± 0.4 | 70.4 ± 6.1 | 11.8 ± 1.2 | 5.5 ± 0.8 | 2.1 ± 0.6 |
| R-41 + PE | 14.7 ± 1.3 | 5.7 ± 0.6 | 60.4 ± 4.2 | 10.3 ± 1.2 | 5.7 ± 0.9 | 2.7 ± 0.2 |
| R-41 + Etn | 15.8 ± 0.9 | 5.5 ± 0.4 | 59.7 ± 3.9 | 10.1 ± 0.9 | 5.9 ± 1.1 | 3.1 ± 0.3 |
Cells were incubated in Ham's F-12 medium containing 10% dialyzed newborn calf serum supplemented with or without either 30 μM PE (R-41 + PE) or 20 μM ethanolamine (R-41 + Etn) at 39.5°C for 3 d. Phospholipids were extracted, separated, and quantified. PE, Phosphatidylethanolamine; PS, phosphatidylserine; PC, phosphatidylcholine; SM, sphingomyelin; PI, phosphatidylinositol. Others include phosphatidylglycerol, phosphatidic acid, and cardiolipin. Data shown are the mean ± SD for three independent experiments.