Phospholipid Compositions of CHO-K1 and R-41 Mutant Cells Cultured in Normal Growth Medium
| . | Percentage of total phospholipids . | |||||
|---|---|---|---|---|---|---|
| Strain . | PE . | PS . | PC . | SM . | PI . | Others . |
| CHO-K1 | 17.6 ± 1.6 | 5.5 ± 0.8 | 58.9 ± 3.8 | 7.8 ± 1.1 | 6.8 ± 0.8 | 3.4 ± 0.4 |
| R-41 | 9.0 ± 0.9 | 5.9 ± 0.5 | 68.8 ± 4.3 | 8.9 ± 1.3 | 5.9 ± 1.2 | 2.1 ± 0.3 |
| . | Percentage of total phospholipids . | |||||
|---|---|---|---|---|---|---|
| Strain . | PE . | PS . | PC . | SM . | PI . | Others . |
| CHO-K1 | 17.6 ± 1.6 | 5.5 ± 0.8 | 58.9 ± 3.8 | 7.8 ± 1.1 | 6.8 ± 0.8 | 3.4 ± 0.4 |
| R-41 | 9.0 ± 0.9 | 5.9 ± 0.5 | 68.8 ± 4.3 | 8.9 ± 1.3 | 5.9 ± 1.2 | 2.1 ± 0.3 |
Cells were incubated in Ham's F-12 medium containing 10% newborn calf serum at 39.5°C for 3 d. Phospholipids were extracted, separated, and quantified. PE, Phosphatidylethanolamine; PS, phosphatidylserine; PC, phosphatidylcholine; SM, sphingomyelin; PI, phosphatidylinositol. Others include phosphatidylglycerol, phosphatidic acid, and cardiolipin. Data shown are the mean ± SD for three independent experiments.