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Table 1

Sites, Transformation Efficiencies, and Gross Phenotypes of Wild Type and Various Mutations on β-Tubulin

Name of variant BTU1 geneAmino acids (positions 437–442)No. of transformants/μg DNAGross phenotype
WT E-E-E-E-G-E 65 normal 
βDEEE440 D-E-E-E-G-E 77 normal 
βEEDE440 E-E-D-E-G-E 89 normal 
βEEED440 E-E-E-D-G-E 120 normal 
BDDEE440 D-D-E-E-G-E 141 normal 
βEEDD440 E-E-D-D-G-E 97 normal 
βEDDD440 E-D-D-D-G-E 68 abnormal 
βDDDE440 D-D-D-E-G-E lethal 
βDDDD440 D-D-D-D-G-E lethal 
βDEDD440 D-E-D-D-G-E lethal 
βDDED440 D-D-E-D-G-E lethal 
βDDED442 E-D-D-D-G-D 18 abnormal 
βEEEEGD442 E-E-E-E-G-D 21 normal 
βDDDDGD442 D-D-D-D-G-D lethal 
no DNA n.a. n.a. 
Name of variant BTU1 geneAmino acids (positions 437–442)No. of transformants/μg DNAGross phenotype
WT E-E-E-E-G-E 65 normal 
βDEEE440 D-E-E-E-G-E 77 normal 
βEEDE440 E-E-D-E-G-E 89 normal 
βEEED440 E-E-E-D-G-E 120 normal 
BDDEE440 D-D-E-E-G-E 141 normal 
βEEDD440 E-E-D-D-G-E 97 normal 
βEDDD440 E-D-D-D-G-E 68 abnormal 
βDDDE440 D-D-D-E-G-E lethal 
βDDDD440 D-D-D-D-G-E lethal 
βDEDD440 D-E-D-D-G-E lethal 
βDDED440 D-D-E-D-G-E lethal 
βDDED442 E-D-D-D-G-D 18 abnormal 
βEEEEGD442 E-E-E-E-G-D 21 normal 
βDDDDGD442 D-D-D-D-G-D lethal 
no DNA n.a. n.a. 
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