Rockefeller University Press

Table 1

Sites, Transformation Efficiencies, and Gross Phenotypes of Wild Type and Various Mutations on β-Tubulin

Name of variant BTU1 gene	Amino acids (positions 437–442)	No. of transformants/μg DNA	Gross phenotype
WT	E-E-E-E-G-E	65	normal
βDEEE₄₄₀	D-E-E-E-G-E	77	normal
βEEDE₄₄₀	E-E-D-E-G-E	89	normal
βEEED₄₄₀	E-E-E-D-G-E	120	normal
BDDEE₄₄₀	D-D-E-E-G-E	141	normal
βEEDD₄₄₀	E-E-D-D-G-E	97	normal
βEDDD₄₄₀	E-D-D-D-G-E	68	abnormal
βDDDE₄₄₀	D-D-D-E-G-E	0	lethal
βDDDD₄₄₀	D-D-D-D-G-E	0	lethal
βDEDD₄₄₀	D-E-D-D-G-E	0	lethal
βDDED₄₄₀	D-D-E-D-G-E	0	lethal
βDDED₄₄₂	E-D-D-D-G-D	18	abnormal
βEEEEGD₄₄₂	E-E-E-E-G-D	21	normal
βDDDDGD₄₄₂	D-D-D-D-G-D	0	lethal
no DNA	n.a.	0	n.a.

Name of variant BTU1 gene	Amino acids (positions 437–442)	No. of transformants/μg DNA	Gross phenotype
WT	E-E-E-E-G-E	65	normal
βDEEE₄₄₀	D-E-E-E-G-E	77	normal
βEEDE₄₄₀	E-E-D-E-G-E	89	normal
βEEED₄₄₀	E-E-E-D-G-E	120	normal
BDDEE₄₄₀	D-D-E-E-G-E	141	normal
βEEDD₄₄₀	E-E-D-D-G-E	97	normal
βEDDD₄₄₀	E-D-D-D-G-E	68	abnormal
βDDDE₄₄₀	D-D-D-E-G-E	0	lethal
βDDDD₄₄₀	D-D-D-D-G-E	0	lethal
βDEDD₄₄₀	D-E-D-D-G-E	0	lethal
βDDED₄₄₀	D-D-E-D-G-E	0	lethal
βDDED₄₄₂	E-D-D-D-G-D	18	abnormal
βEEEEGD₄₄₂	E-E-E-E-G-D	21	normal
βDDDDGD₄₄₂	D-D-D-D-G-D	0	lethal
no DNA	n.a.	0	n.a.