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Table 1

Proteolytic Processing of Nuclear Transport Components during Apoptosis

ProteinMCF-7 cellsMCF-7 cells expressing caspase-3
Soluble components  
 Importin β1 NC NC 
 Transportin NC NC 
 Ran NC NC 
 RanBP1 NC NC 
 RanGAP1 NC 
 RCC1 NC NC 
 NTF2 NC NC 
 CAS NC NC 
Nuclear pore components  
 p62 NC NC 
 p153 ND NC 
 p175 NC NC 
 p214 NC NC 
 p270 NC 
ProteinMCF-7 cellsMCF-7 cells expressing caspase-3
Soluble components  
 Importin β1 NC NC 
 Transportin NC NC 
 Ran NC NC 
 RanBP1 NC NC 
 RanGAP1 NC 
 RCC1 NC NC 
 NTF2 NC NC 
 CAS NC NC 
Nuclear pore components  
 p62 NC NC 
 p153 ND NC 
 p175 NC NC 
 p214 NC NC 
 p270 NC 

MCF-7 and MCF-7/C3 were either left untreated or were treated with 50 μM cisplatin for 12 h, which resulted in >75% apoptotic cells as scored by chromatin condensation. Cell lysates were analyzed by immunoblotting using monoclonal antibodies to nuclear transport proteins. NC, not cleaved; C, cleaved; ND, not detected.

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