Motility, Dynamic Morphology, and Chemotaxis Parameters of Control, torA−, and Mek1/torA− Cells Translocating in Buffer and a Spatial Gradient of cAMP
| Condition . | Cell type . | Cell number . | Instantaneous velocity . | Directional change . | Maximum length . | Area . | Roundness . | Chemotactic index . |
|---|---|---|---|---|---|---|---|---|
| μm/min | °/min | μm | μm2 | % | ||||
| Buffer | Control | 35 | 15.2 ± 4.2 | 29.7 ± 10.6 | 24.0 ± 4.3 | 108 ± 28 | 52.6 ± 11.9 | — |
| Buffer | torA− | 28 | 5.2 ± 2.0 | 50.7 ± 12.7 | 19.0 ± 3.5 | 105 ± 24 | 50.8 ± 9.1 | — |
| Buffer | Mek1/torA− | 24 | 8.0 ± 2.6 | 38.9 ± 10.2 | 22.6 ± 4.2 | 110 ± 21 | 53.8 ± 6.7 | — |
| Spatial gradient | Control | 53 | 11.1 ± 3.9 | 23.8 ± 15.4 | 21.2 ± 5.0 | 115 ± 29 | 31.4 ± 6.4 | +0.60 ± 0.33 |
| Spatial gradient | torA− | 40 | 7.8 ± 2.0 | 35.7 ± 10.4 | 20.2 ± 2.6 | 125 ± 34 | 41.3 ± 11.2 | +0.12 ± 0.39 |
| Spatial gradient | Mek1/torA− | 32 | 7.5 ± 3.2 | 25.3 ± 11.6 | 20.0 ± 2.3 | 119 ± 19 | 39.2 ± 6.7 | +0.52 ± 0.34 |
| Condition . | Cell type . | Cell number . | Instantaneous velocity . | Directional change . | Maximum length . | Area . | Roundness . | Chemotactic index . |
|---|---|---|---|---|---|---|---|---|
| μm/min | °/min | μm | μm2 | % | ||||
| Buffer | Control | 35 | 15.2 ± 4.2 | 29.7 ± 10.6 | 24.0 ± 4.3 | 108 ± 28 | 52.6 ± 11.9 | — |
| Buffer | torA− | 28 | 5.2 ± 2.0 | 50.7 ± 12.7 | 19.0 ± 3.5 | 105 ± 24 | 50.8 ± 9.1 | — |
| Buffer | Mek1/torA− | 24 | 8.0 ± 2.6 | 38.9 ± 10.2 | 22.6 ± 4.2 | 110 ± 21 | 53.8 ± 6.7 | — |
| Spatial gradient | Control | 53 | 11.1 ± 3.9 | 23.8 ± 15.4 | 21.2 ± 5.0 | 115 ± 29 | 31.4 ± 6.4 | +0.60 ± 0.33 |
| Spatial gradient | torA− | 40 | 7.8 ± 2.0 | 35.7 ± 10.4 | 20.2 ± 2.6 | 125 ± 34 | 41.3 ± 11.2 | +0.12 ± 0.39 |
| Spatial gradient | Mek1/torA− | 32 | 7.5 ± 3.2 | 25.3 ± 11.6 | 20.0 ± 2.3 | 119 ± 19 | 39.2 ± 6.7 | +0.52 ± 0.34 |