Table 1

Fluorescent Labels Used in Experiment in Fig. 2 

Fluorescent labelTarget in cellPurpose
DAPI Nucleus, kDNA To identify the kDNA network; fluorescence intensity is proportional to extent of kDNA replication 
TdT-incorporated TR-dUTP Minicircles (free or network bound) that contain gaps To label minicircles that have undergone replication 
FISH with fluorescein-labeled minicircle probe Minicircle replication intermediates with single- stranded regions (Englund et al. 1982) To identify the location of free minicircle replication intermediates 
Fluorescent labelTarget in cellPurpose
DAPI Nucleus, kDNA To identify the kDNA network; fluorescence intensity is proportional to extent of kDNA replication 
TdT-incorporated TR-dUTP Minicircles (free or network bound) that contain gaps To label minicircles that have undergone replication 
FISH with fluorescein-labeled minicircle probe Minicircle replication intermediates with single- stranded regions (Englund et al. 1982) To identify the location of free minicircle replication intermediates 
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