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Table III.

Colocalization of HA with KIFC3 and discolocalization of cytoplasmic dynein with KIFC3 on the vesicles in apical cytoplasm


Label

 
HA only (10 nm)a
 
KIFC3 only (0.4 nm)a
 
HA + KIFC3
 
No label
 
Observedb 93 49 9c 1198 
Expectedd (97) (54) (4.4) (1193) 

 
DIC onlya
 
KIFC3 only
 
DIC 1 KIFC3
 
No label
 
Observedb 87 74 2e 734 
Expectedd (81) (69) (7.5) (740) 

 
Control (10 nm)
 
0.4 nm
 
Both (10 1 0.4 nm)
 
No label
 
Observedb 526 
Expectedd
 
(5)
 
(8)
 
(0.1)
 
(526)
 

Label

 
HA only (10 nm)a
 
KIFC3 only (0.4 nm)a
 
HA + KIFC3
 
No label
 
Observedb 93 49 9c 1198 
Expectedd (97) (54) (4.4) (1193) 

 
DIC onlya
 
KIFC3 only
 
DIC 1 KIFC3
 
No label
 
Observedb 87 74 2e 734 
Expectedd (81) (69) (7.5) (740) 

 
Control (10 nm)
 
0.4 nm
 
Both (10 1 0.4 nm)
 
No label
 
Observedb 526 
Expectedd
 
(5)
 
(8)
 
(0.1)
 
(526)
 
a

HA and KIFC3 were labeled as shown in the legend to Fig. 6 D. DIC was labeled by 10-nm gold antibody.

b

Numbers of vesicles labeled with indicated antibodies.

c

P < 0.01 by χ2 test.

d

Expectancy calculated on assumption of the independent distribution of the two molecules on vesicles.

e

P < 0.01 by χ2 test.

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