Strains used in this study
Strain . | Genotype . | Source, derivation, or reference . |
|---|---|---|
| W303 | Mat a/α ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3, 112 can 1-100/112 can1-100 | |
| DF5a | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 | |
| DF5α | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 | |
| DF5 | Mata/α ura3-52/ura3-52 lys2-801/52/lys2-801 his3-200/his3-200 leu2-3,112/leu2-3,112 trp1-1/trp1-1 nup157Δ::URA3 | |
| NP157-2.1 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 | Aitchison et al.,1995a |
| NP120-25-3 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup120Δ::URA3 | Aitchison et al.,1995a |
| NP120-25-4 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup120Δ::URA3 | Aitchison et al.,1995a |
| NP53/NP59 | Matα ade2 ura3 his3 trp1 leu2 nup53Δ::HIS3 nup59Δ::HIS3 | Marelli et al., 1998 |
| NP53-B1 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup53Δ::HIS3 | Marelli et al., 1998 |
| NP59-23 | Mataade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup59Δ::HIS3 | Marelli et al., 1998 |
| NP170pA | Mata ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 Nup170-protA (URA3-HIS3)/+ | Rout et al., 2000 |
| NP157pA | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 Nup157-protA (URA3-HIS3)/+ | Rout et al., 2000 |
| NP60pA | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 Nup60-protA (HIS3)/+ | Rout et al., 2000 |
| M1GFP | Matα ura3-52 lys2-801 ade2-101 his3Δ200 leu2Δ1 MAD1-GFP/HIS5 | This work |
| M2GFP | Matα ura3-52 lys2-801 ade2-101 his3Δ200 leu2Δ1 MAD2-GFP/HIS5 | This work |
| YMB1296 | Matα ura3-52 lys2-801 ade2-101 his3Δ200 leu2Δ1 MAD2-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB1299 | Matα ura3-52 lys2-801 ade2-101 his3Δ200 leu2Δ1 MAD1-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB1451 | Mataura3-52 lys2-801 ade2-101 his3Δ200 trp1Δ1 nup170Δ::KAN | This work |
| YMB1482 | Matα ura3-52 lys2-801 his3Δ-200 leu2-3,112 trp1-1 nup170Δ::KAN | This work |
| YMB1900 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 mad2Δ::HIS3 [pMAD2/URA3] | This work |
| YMB1906 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 mad2Δ::HIS3 | This work |
| YMB1908 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 mad1Δ::KAN | This work |
| YMB1911 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 mad1Δ::KAN [pMAD1/URA3] | This work |
| YMB1979 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup170Δ::HIS3 mad1Δ::KAN | Segregant of sporulated YMB1482/1911 |
| YMB2008 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup170Δ::HIS3 mad2Δ::HIS3 | Segregant of sporulated YMB1482/1900 |
| YMB2018 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 MAD1-GFP/HIS5 | This work |
| YMB2020 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 MAD1-GFP/HIS5 | This work |
| YMB2022 | Mataura3 his3 nup53Δ::HIS3 mad2Δ::HIS | Segregant of sporulated NP53/NP15782/YMB1906 |
| YMB2029 | Matα ura3 his3 nup157Δ::URA3 mad2Δ::HIS3 | Segregant of sporulated NP53/NP1578-2/YMB1906 |
| YMB2032 | Matα ura3 his3 nup157Δ::URA3 mad1Δ::KAN | Segregant of sporulated NP53/NP1578-3/YMB1908 |
| YMB2035 | Mataura3 his3 nup53Δ::HIS3 mad1Δ::KAN | Segregant of sporulated NP53/NP1578-3/YMB1908 |
| YMB2064 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup170Δ::HIS3 MAD1-GFP/HIS5 | Segregant of sporulated YMB1482/YMB2020 |
| YMB2067 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup53Δ::HIS3 MAD1-GFP/HIS5 | Segregant of sporulated NP53-B1/YMB2018 |
| YMB2081 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup157Δ::URA3 MAD1-GFP/HIS5 | Segregant of sporulated NP157-2.1/YMB2018 |
| YMB2086 | Matα ura3-52 lys2-801 ade2-101 his3-200 leu2Δ1 nup170Δ::KAN MAD2-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB2087 | Matα ura3-52 lys2-801 ade2-101 his3-200 leu2Δ1 nup53Δ::KAN MAD2-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB2088 | Matα ura3-52 lys2-801 ade2-101 his3-200 leu2Δ1 mad1Δ::KAN MAD2-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB3048 | Mataura his3 trp1-1 leu2-3,112 nup59Δ::HIS3 mad2Δ::HIS3 | Segregant of sporulated NP59-23/YMB1906 |
| YMB3051 | Mataura his3 trp1-1 leu2-3,112 nup59Δ::HIS3 mad2Δ::KAN | Segregant of sporulated NP59-23/YMB1908 |
| NP120M1G | Matα ura3 his3 trp1 leu2 nup120Δ::URA3 MAD1-GFP/HIS5 | Segregant of sporulated NP120-25-3/YMB2020 |
| NP120M2G | Matα ura3 his3 trp1 leu2 nup120Δ::URA3 MAD1-GFP/HIS5 | Segregant of sporulated NP120-25-4/YMB1296 |
| YPH278 | Matα ura3-52 lys2-801 ade2-101 his3-200 leu2Δ1 CFIII (CEN3L.YPH278)URA3 SUP11 | Spencer et al., 1990 |
Strain . | Genotype . | Source, derivation, or reference . |
|---|---|---|
| W303 | Mat a/α ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3, 112 can 1-100/112 can1-100 | |
| DF5a | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 | |
| DF5α | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 | |
| DF5 | Mata/α ura3-52/ura3-52 lys2-801/52/lys2-801 his3-200/his3-200 leu2-3,112/leu2-3,112 trp1-1/trp1-1 nup157Δ::URA3 | |
| NP157-2.1 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 | Aitchison et al.,1995a |
| NP120-25-3 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup120Δ::URA3 | Aitchison et al.,1995a |
| NP120-25-4 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup120Δ::URA3 | Aitchison et al.,1995a |
| NP53/NP59 | Matα ade2 ura3 his3 trp1 leu2 nup53Δ::HIS3 nup59Δ::HIS3 | Marelli et al., 1998 |
| NP53-B1 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup53Δ::HIS3 | Marelli et al., 1998 |
| NP59-23 | Mataade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup59Δ::HIS3 | Marelli et al., 1998 |
| NP170pA | Mata ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 Nup170-protA (URA3-HIS3)/+ | Rout et al., 2000 |
| NP157pA | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 Nup157-protA (URA3-HIS3)/+ | Rout et al., 2000 |
| NP60pA | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 Nup60-protA (HIS3)/+ | Rout et al., 2000 |
| M1GFP | Matα ura3-52 lys2-801 ade2-101 his3Δ200 leu2Δ1 MAD1-GFP/HIS5 | This work |
| M2GFP | Matα ura3-52 lys2-801 ade2-101 his3Δ200 leu2Δ1 MAD2-GFP/HIS5 | This work |
| YMB1296 | Matα ura3-52 lys2-801 ade2-101 his3Δ200 leu2Δ1 MAD2-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB1299 | Matα ura3-52 lys2-801 ade2-101 his3Δ200 leu2Δ1 MAD1-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB1451 | Mataura3-52 lys2-801 ade2-101 his3Δ200 trp1Δ1 nup170Δ::KAN | This work |
| YMB1482 | Matα ura3-52 lys2-801 his3Δ-200 leu2-3,112 trp1-1 nup170Δ::KAN | This work |
| YMB1900 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 mad2Δ::HIS3 [pMAD2/URA3] | This work |
| YMB1906 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 mad2Δ::HIS3 | This work |
| YMB1908 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 mad1Δ::KAN | This work |
| YMB1911 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 mad1Δ::KAN [pMAD1/URA3] | This work |
| YMB1979 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup170Δ::HIS3 mad1Δ::KAN | Segregant of sporulated YMB1482/1911 |
| YMB2008 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup170Δ::HIS3 mad2Δ::HIS3 | Segregant of sporulated YMB1482/1900 |
| YMB2018 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 MAD1-GFP/HIS5 | This work |
| YMB2020 | Mataura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 MAD1-GFP/HIS5 | This work |
| YMB2022 | Mataura3 his3 nup53Δ::HIS3 mad2Δ::HIS | Segregant of sporulated NP53/NP15782/YMB1906 |
| YMB2029 | Matα ura3 his3 nup157Δ::URA3 mad2Δ::HIS3 | Segregant of sporulated NP53/NP1578-2/YMB1906 |
| YMB2032 | Matα ura3 his3 nup157Δ::URA3 mad1Δ::KAN | Segregant of sporulated NP53/NP1578-3/YMB1908 |
| YMB2035 | Mataura3 his3 nup53Δ::HIS3 mad1Δ::KAN | Segregant of sporulated NP53/NP1578-3/YMB1908 |
| YMB2064 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup170Δ::HIS3 MAD1-GFP/HIS5 | Segregant of sporulated YMB1482/YMB2020 |
| YMB2067 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup53Δ::HIS3 MAD1-GFP/HIS5 | Segregant of sporulated NP53-B1/YMB2018 |
| YMB2081 | Matα ura3-52 lys2-801 his3-200 leu2-3,112 trp1-1 nup157Δ::URA3 MAD1-GFP/HIS5 | Segregant of sporulated NP157-2.1/YMB2018 |
| YMB2086 | Matα ura3-52 lys2-801 ade2-101 his3-200 leu2Δ1 nup170Δ::KAN MAD2-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB2087 | Matα ura3-52 lys2-801 ade2-101 his3-200 leu2Δ1 nup53Δ::KAN MAD2-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB2088 | Matα ura3-52 lys2-801 ade2-101 his3-200 leu2Δ1 mad1Δ::KAN MAD2-GFP/HIS5 CFIII (CEN3L.YPH278)URA3 SUP11 | This work |
| YMB3048 | Mataura his3 trp1-1 leu2-3,112 nup59Δ::HIS3 mad2Δ::HIS3 | Segregant of sporulated NP59-23/YMB1906 |
| YMB3051 | Mataura his3 trp1-1 leu2-3,112 nup59Δ::HIS3 mad2Δ::KAN | Segregant of sporulated NP59-23/YMB1908 |
| NP120M1G | Matα ura3 his3 trp1 leu2 nup120Δ::URA3 MAD1-GFP/HIS5 | Segregant of sporulated NP120-25-3/YMB2020 |
| NP120M2G | Matα ura3 his3 trp1 leu2 nup120Δ::URA3 MAD1-GFP/HIS5 | Segregant of sporulated NP120-25-4/YMB1296 |
| YPH278 | Matα ura3-52 lys2-801 ade2-101 his3-200 leu2Δ1 CFIII (CEN3L.YPH278)URA3 SUP11 | Spencer et al., 1990 |