Viability of wild-type and mutant strains upon dicentric chromosome activation
Relevant genotype . | RAD 52 . | % Viabilitya . |
|---|---|---|
| WT | + | 66.0 ± 1.0 |
| rad52Δ | − | 2.0 ± 0.2 |
| ndc10-2ts (33°C)b | + | 74.5 ± 1.0 |
| ndc10-2tsrad52Δ (33°C)b | − | 36.0 ± 2.0 |
| mcm21Δ | + | 78.0 ± 1.0 |
| mcm21Δ rad52Δ | − | 31.0 ± 10.0 |
| mcm16 | + | 80.0 ± 2.0 |
| mcm16Δ rad52Δ | − | 43.0 ± 9.0 |
| iml3Δ | + | 81.0 ± 6.0 |
| iml3Δ rad52Δ | − | 58.0 ± 3.0 |
| ctf19Δ | + | 82.0 ± 3.0 |
| ctf19Δ rad52Δ | − | 62.0 ± 4.0 |
| ch14Δ | + | 81.0 ± 2.0 |
| ch14Δ rad52Δ | − | 80.0 ± 7.0 |
Relevant genotype . | RAD 52 . | % Viabilitya . |
|---|---|---|
| WT | + | 66.0 ± 1.0 |
| rad52Δ | − | 2.0 ± 0.2 |
| ndc10-2ts (33°C)b | + | 74.5 ± 1.0 |
| ndc10-2tsrad52Δ (33°C)b | − | 36.0 ± 2.0 |
| mcm21Δ | + | 78.0 ± 1.0 |
| mcm21Δ rad52Δ | − | 31.0 ± 10.0 |
| mcm16 | + | 80.0 ± 2.0 |
| mcm16Δ rad52Δ | − | 43.0 ± 9.0 |
| iml3Δ | + | 81.0 ± 6.0 |
| iml3Δ rad52Δ | − | 58.0 ± 3.0 |
| ctf19Δ | + | 82.0 ± 3.0 |
| ctf19Δ rad52Δ | − | 62.0 ± 4.0 |
| ch14Δ | + | 81.0 ± 2.0 |
| ch14Δ rad52Δ | − | 80.0 ± 7.0 |
Mean ± SD.
The ndc10-2ts dicentric strains were grown at 24°C and plated out at 33°C (semipermissive for growth) on glucose and galactose plates.
Strains containing GALCEN3 in wild type, chl4Δ, iml3Δ, mcm21Δ, and ndc10-2, in the presence or absence of RAD52 (indicated by + or −), respectively, were plated for single colonies on galactose and glucose. Dicentric viability is a measure of colony forming units on glucose/galactose. 48% of ndc10-2 rad52Δ (36% viability in rad52Δ/75% in RAD52), 40% of mcm21Δ rad52Δ (31% in rad52Δ/78% in RAD52), 55% of mcm16Δ rad52Δ (43% in rad52Δ/80% in RAD52), 70% of iml3Δ rad52Δ (58% in rad52Δ/81% in RAD52), 75% of ctf19Δ rad52Δ (62% in rad52Δ/ 82% in RAD52), and 100% of chl4Δ rad52Δ (80% in rad52Δ/81% in RAD52) cells were viable. n = 10 determinants for chl4Δ, iml3Δ, and mcm21Δ; n = 5 determinants for ctf19Δ and mcm16Δ; n = 6 determinants for ndc10-2ts; and n = 4 determinants for wild type (WT).