Persistence of the stably inherited state in the absence of Chl4p
Relevant genotype . | % Mitotic stability . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| 0–20 | 20–40 | 40–60 | 60–85 | ||||
| Wild type (35 generations) | 14/25 (52 ± 6) | 11/25 (70 ± 7) | |||||
| GAL–UBI–CHL4 | |||||||
| Glu→Glu | 25/25 (9 ± 7) | ||||||
| Gal→Glu | 34/66 (12 ± 5) | 24/66 (27 ± 6) | 8/66 (48 ± 5) | ||||
Relevant genotype . | % Mitotic stability . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| 0–20 | 20–40 | 40–60 | 60–85 | ||||
| Wild type (35 generations) | 14/25 (52 ± 6) | 11/25 (70 ± 7) | |||||
| GAL–UBI–CHL4 | |||||||
| Glu→Glu | 25/25 (9 ± 7) | ||||||
| Gal→Glu | 34/66 (12 ± 5) | 24/66 (27 ± 6) | 8/66 (48 ± 5) | ||||
Centromere plasmid transformed into either wild-type or GAL–UBI–CHL4 strains on glucose were grown in YPD for 8–10 h and plated on YPD plates. After 3–4 d, colonies were isolated and grown in selective glucose media for 10 h and plated on YPD. Colonies were counted after 3–4 d, n = 25 colonies purified. GAL–UBI–CHL4 cells transformed with centromere plasmid on galactose were washed, shifted to YPD for 8–10 h, and plated on YPD, n = 66 colonies purified. Mitotic stability was determined using the colony color assay as described in the Materials and methods.