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Table I.

Purification of CCP MT–depolymerizing activity fromXenopusegg extract


Step

Total activity

Protein

Specific activity

Fold purification

 
U
 
μg
 
U/μg
 

 
HSS 440,000 1,320,000 0.33 1.00 
AS supe 400,000 524,000 0.76 2.30 
Phenyl sepharose 300,000 122,000 2.45 7.40 
Heparin 150,000 2,410 62.30 188.79 
MonoS 1 72,000 595 121.00 366.67 
Superose 6 15,000 65 230.77 699.30 
MonoQ FT 10,000 28 357.14 1,082.24 
MonoS 2
 
4,500
 
5
 
900.00
 
2,727.27
 

Step

Total activity

Protein

Specific activity

Fold purification

 
U
 
μg
 
U/μg
 

 
HSS 440,000 1,320,000 0.33 1.00 
AS supe 400,000 524,000 0.76 2.30 
Phenyl sepharose 300,000 122,000 2.45 7.40 
Heparin 150,000 2,410 62.30 188.79 
MonoS 1 72,000 595 121.00 366.67 
Superose 6 15,000 65 230.77 699.30 
MonoQ FT 10,000 28 357.14 1,082.24 
MonoS 2
 
4,500
 
5
 
900.00
 
2,727.27
 

Activity was defined as the maximal dilution that would depolymerize all microtubules in the visual assay within 10 min (see Materials and methods). HSS, high-speed supernatant; AS supe, 40% AS supernatant.

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