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Table I.

Time spent in prometaphase by PtK1 cells treated with Hesperadin


PtK1 cells

NEB to last chromosome congressing

NEB to anaphase onset

No. of monooriented chromosomes at anaphase onset

n

 
min:sec
 
min:sec
 

 

 
Controla 13:19 (08:30–21:14) 23:12 (14:00–50:45) 16 
Hesperadina
 
NA
 
16:27 (10:30–23:46)
 
3.6 (1–6)
 
23
 

PtK1 cells

NEB to last chromosome congressing

NEB to anaphase onset

No. of monooriented chromosomes at anaphase onset

n

 
min:sec
 
min:sec
 

 

 
Controla 13:19 (08:30–21:14) 23:12 (14:00–50:45) 16 
Hesperadina
 
NA
 
16:27 (10:30–23:46)
 
3.6 (1–6)
 
23
 
a

PtK1 cells were left untreated or were treated with 500 nM Hesperadin, and followed by video microscopy in a 37°C warm room.

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