Summary of movement characteristics of GFP–NFHala and GFP–NFHasp filaments in living cortical neurons
Velocity of moving GFP–NFHala and GFP–NFHasp excluding pauses . | No difference between GFP–NFHala and GFP–NFHasp. GFP–NFHala varied between 0.08 and 1.13 μm/s in the anterograde direction and between 0.08 and 1.27 μm/s in the retrograde direction; GFP–NFHasp varied between 0.08 and 1.1 μm/s in the anterograde direction and between 0.07 and 1.22 μm/s in the retrograde direction (analyzed by Mann-Whitney test). . |
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| Anterograde vs. retrograde movement of GFP–NFHala and GFP–NFHasp | No difference between GFP–NFHala and GFP–NFHasp (analyzed by chi-square test). |
| Pauses in movement of moving GFP–NFHala and GFP–NFHasp | GFP–NFHala pauses significantly less than GFP–NFHasp (16 vs. 37%; P = 0.005 by Mann-Whitney test). |
| Distance moved by GFP–NFHala and GFP–NFHasp per minute of microscope observation time | GFP–NFHala moves significantly more than GFP–NFHasp. GFP–NFHala moved a total of 8.64 μm/min of observation, whereas GFP–NFHasp moved 3.12 μm/min of observation (P = 0.001 by Mann-Whitney test). |
Velocity of moving GFP–NFHala and GFP–NFHasp excluding pauses . | No difference between GFP–NFHala and GFP–NFHasp. GFP–NFHala varied between 0.08 and 1.13 μm/s in the anterograde direction and between 0.08 and 1.27 μm/s in the retrograde direction; GFP–NFHasp varied between 0.08 and 1.1 μm/s in the anterograde direction and between 0.07 and 1.22 μm/s in the retrograde direction (analyzed by Mann-Whitney test). . |
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| Anterograde vs. retrograde movement of GFP–NFHala and GFP–NFHasp | No difference between GFP–NFHala and GFP–NFHasp (analyzed by chi-square test). |
| Pauses in movement of moving GFP–NFHala and GFP–NFHasp | GFP–NFHala pauses significantly less than GFP–NFHasp (16 vs. 37%; P = 0.005 by Mann-Whitney test). |
| Distance moved by GFP–NFHala and GFP–NFHasp per minute of microscope observation time | GFP–NFHala moves significantly more than GFP–NFHasp. GFP–NFHala moved a total of 8.64 μm/min of observation, whereas GFP–NFHasp moved 3.12 μm/min of observation (P = 0.001 by Mann-Whitney test). |
GFP–NFHala movement was characterized from movies acquired from 27 cells, with the duration of observation ranging from 1 to 2.83 min; GFP–NFHasp movement was from 17 cells, with the duration of observation ranging from 1 to 2.83 min. Calculations of distance moved per minute of observation time were made from 37 GFP–NFHala- and 26 GFP–NFHasp-transfected cells viewed for a total of 60 and 40.42 min, respectively.