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Table I.

Subcellular distribution of full-length and various GFP-tagged NuSAP fragments in (non)permeabilized cells


Construct

Subcellular localization

Localization after permeabilization

Microtubule bundling

Cytoplasm
Nucleus
Nucleolus
Microtubules
Nucleus
Nucleolus

1–139 ++ +++ ++ 
141–240 ++ 
243–427 ++++ +++++ ++ ++ +++ 
129–367 ++ ++ +++ 
368–427 ++++ 
1–427 ++++ +++++ ++ ++ +++ 
Empty
 
++
 
+
 
-
 
-
 
-
 
-
 
-
 

Construct

Subcellular localization

Localization after permeabilization

Microtubule bundling

Cytoplasm
Nucleus
Nucleolus
Microtubules
Nucleus
Nucleolus

1–139 ++ +++ ++ 
141–240 ++ 
243–427 ++++ +++++ ++ ++ +++ 
129–367 ++ ++ +++ 
368–427 ++++ 
1–427 ++++ +++++ ++ ++ +++ 
Empty
 
++
 
+
 
-
 
-
 
-
 
-
 
-
 

Presence and intensity of GFP fluorescence is indicated together with the microtubule-bundling potential of the different fusion proteins.

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