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Table I.

Invertase secretion in wild-type andmsb3Δ msb4Δcells


Induction time (min)

0

15

30

45

60

90

120
YEF473A (WT)        
Ext 0.02 0.02 0.04 0.09 0.12 0.16 0.20 
Int 0.02 0.03 0.03 0.02 0.02 0.02 0.02 
Ext/(Ext+Int) 0.50 0.40 0.57 0.82 0.86 0.89 0.91 
YEF1289 (msb3Δ msb4Δ       
Ext 0.02 0.02 0.03 0.12 0.20 0.35 0.49 
Int 0.02 0.03 0.03 0.06 0.06 0.06 0.08 
Ext/(Ext+Int)
 
0.50
 
0.40
 
0.50
 
0.67
 
0.77
 
0.85
 
0.86
 

Induction time (min)

0

15

30

45

60

90

120
YEF473A (WT)        
Ext 0.02 0.02 0.04 0.09 0.12 0.16 0.20 
Int 0.02 0.03 0.03 0.02 0.02 0.02 0.02 
Ext/(Ext+Int) 0.50 0.40 0.57 0.82 0.86 0.89 0.91 
YEF1289 (msb3Δ msb4Δ       
Ext 0.02 0.02 0.03 0.12 0.20 0.35 0.49 
Int 0.02 0.03 0.03 0.06 0.06 0.06 0.08 
Ext/(Ext+Int)
 
0.50
 
0.40
 
0.50
 
0.67
 
0.77
 
0.85
 
0.86
 

Invertase activity (in units) was measured at different time points after induction at 24°C. Ext and Int stand for external (secreted) and internal pools of invertase, respectively.

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