Rockefeller University Press

Table I.

Ensemble properties of myosin

Construct	NH₄⁺-ATPase	Actin-activated ATPase			Actin velocity^{^b}
		K_m	V_max
	s⁻¹	μM	s⁻¹	μms⁻¹
wt-HMM^{^a}	24.2 ± 2.8	20 ± 4	2.5 ± 0.2	1.6 ± 0.2 (58)
E470A-HMM^{^a}	0.01	ND	ND	0.00
E470A/wt-HMM^{^a}	12.0 ± 0.8	27 ± 7	1.8 ± 0.2	1.5 ± 0.2 (61)
S1-neo	ND	100 ± 44	1.8 ± 0.5	0.5 ± 0.1 (17)

Construct	NH₄⁺-ATPase	Actin-activated ATPase			Actin velocity^{^b}
		K_m	V_max
	s⁻¹	μM	s⁻¹	μms⁻¹
wt-HMM^{^a}	24.2 ± 2.8	20 ± 4	2.5 ± 0.2	1.6 ± 0.2 (58)
E470A-HMM^{^a}	0.01	ND	ND	0.00
E470A/wt-HMM^{^a}	12.0 ± 0.8	27 ± 7	1.8 ± 0.2	1.5 ± 0.2 (61)
S1-neo	ND	100 ± 44	1.8 ± 0.5	0.5 ± 0.1 (17)

^a

Values obtained from Rovner et al. (2003).

^b

Numbers in brackets denote number of filaments sampled.

ND, not determined.