Table II.

Single molecule properties of myosin


Construct

Step sizea

Step durationb

nm
ms
wt-HMM 10.2 ± 0.6 (19) 104 ± 13 (15) 
S1-neo 4.4 ± 0.7 (10) 189 ± 37 (10) 
E470A/wt-HMM
 
10.4 ± 0.6 (11)
 
106 ± 10 (9)
 

Construct

Step sizea

Step durationb

nm
ms
wt-HMM 10.2 ± 0.6 (19) 104 ± 13 (15) 
S1-neo 4.4 ± 0.7 (10) 189 ± 37 (10) 
E470A/wt-HMM
 
10.4 ± 0.6 (11)
 
106 ± 10 (9)
 
a

ANOVA test indicates wt-HMM and S1-neo step sizes are statistically different, whereas wt-HMM and E470A/wt-HMM are not.

b

[ATP] = 10 μm.

Numbers in brackets denote number of single molecules sampled. Errors are given as SEM values.

Close Modal

or Create an Account

Close Modal
Close Modal