Percentage of cells exhibiting dispersed γ-adaptin following expression of activated Rab8Q67L
Cell type . | AP-1 phenotype . | Rab8 expressed . | TGN organization (percentage of transfected cells) . | . | . | ||
|---|---|---|---|---|---|---|---|
. | . | . | Dispersed . | Not dispersed . | Other . | ||
| LLC-PK1 | AP-1A+/AP-1B− | Rab8Q67L | 0 | 76 | 25 | ||
| AP-1A+/AP-1B+ | Rab8Q67L | 60 | 14 | 23 | |||
| EFA | AP-1A−/AP-1B+ | GFP alone | 7 | 93 | ND | ||
| AP-1A−/AP-1B+ | GFP-Rab8Q67L | 79 | 18 | ND | |||
Cell type . | AP-1 phenotype . | Rab8 expressed . | TGN organization (percentage of transfected cells) . | . | . | ||
|---|---|---|---|---|---|---|---|
. | . | . | Dispersed . | Not dispersed . | Other . | ||
| LLC-PK1 | AP-1A+/AP-1B− | Rab8Q67L | 0 | 76 | 25 | ||
| AP-1A+/AP-1B+ | Rab8Q67L | 60 | 14 | 23 | |||
| EFA | AP-1A−/AP-1B+ | GFP alone | 7 | 93 | ND | ||
| AP-1A−/AP-1B+ | GFP-Rab8Q67L | 79 | 18 | ND | |||
The numbers of cells with TGN disruption were counted in cells of each genotype, AP-1A only (AP-1A+/AP-1B−), AP-1A+/AP-1B+, and AP-1B only (AP-1A−/AP-1B+) in the presence of GFP-Rab8Q67L or GFP vector alone. The SEM for Rab8Q67L expressed in LLC-PK1 AP-1A+/AP-1B− and AP-1A+/AP-1B+ was 13 and 20%, respectively. The SEM for the EFA cells expressing GFP alone or GFP-Rab8Q67L was 12 and 15%, respectively. The SEM was calculated as the square root of n (n = number of cells). EFA, embryonic fibroblast cells; ND, not determined.