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Table I.

Yeast strains


Strain

Genotype
VG982-6Aa Mata trp1 ura3 bar 
VG986-5Ba pds5-1 
VG987-5Da pds5-2 
VG988-1Ca pds5-3 
VG985-7Ca mcd1-1 
VG2029-7Ba smc2-8 
VG2014-4Da top2-4 
VG971-1Aa pds1Δ::LEU2 leu2 
VG2128-2Da pds5-1 his3 
VG2066-7Ba PDS5-6MYC 
VG2424-4Ca PDS5-6MYC cdc15-2 
VG2067-2Ba PDS5-12MYC lys2 
VG1360-7C Matα smc1-2:LEU2 ura3 leu2 bar1 
K5832b Mata mcd1-73/scc1-73 ade2 can1 leu2 his3 ura3 
K6013b smc1-259 trp1 
K5824b smc3-42 trp 
K5828b scc2-4 trp1 
2788 Mata esp1-1 ura3 leu2 can1 lys2 
VG2524-1A Mata PDS5-6MYC pGAL-TRP1:trp1 leu2 ura3 his3 bar1 lys2 
VG2525-2B Mata PDS5-6MYC pGAL-SMT4-TRP1:trp1 leu2 ura3 lys2 his3 bar1 
VG2465-4D Mata PDS5-6MYC smt4::HIS3 leu2 his3 bar1 
VG2463-1D Mata smt4::HIS3 leu2 his3 bar1 
VG2390-37Ac Mata TETGFP-LEU2:leu2 TetOx224-URA3:ura3 trp1 bar1 
VG2416-12Ac pds5-2 
VG2452-7Ac pGAL-TRP1:trp1 
VG2450-7Ac PDS1-3HA-KAN 
VG2456-5Cc pds5-2 PDS1-3HA-KAN 
VG2445-5Bc pds5-2 pGAL-SMT4-TRP1:trp 
VG2446-6Ac pds5-2 pGAL-TRP1:trp1 
VG2452-7Ac pGAL-TRP1:trp1 
VG2485-3Bc pds5-2 pGAL1-SMT4-TRP1:trp1 PDS1-3HA-KAN 
VG2486-14Ac pds5-2 pGAL1-TRP1:trp1 PDS1-3HA-KAN 
VG2259-6B
 
Mata pds5::URA3 trp1 leu2 ura3 + pTH10 (PDS5 CEN LEU2)
 

Strain

Genotype
VG982-6Aa Mata trp1 ura3 bar 
VG986-5Ba pds5-1 
VG987-5Da pds5-2 
VG988-1Ca pds5-3 
VG985-7Ca mcd1-1 
VG2029-7Ba smc2-8 
VG2014-4Da top2-4 
VG971-1Aa pds1Δ::LEU2 leu2 
VG2128-2Da pds5-1 his3 
VG2066-7Ba PDS5-6MYC 
VG2424-4Ca PDS5-6MYC cdc15-2 
VG2067-2Ba PDS5-12MYC lys2 
VG1360-7C Matα smc1-2:LEU2 ura3 leu2 bar1 
K5832b Mata mcd1-73/scc1-73 ade2 can1 leu2 his3 ura3 
K6013b smc1-259 trp1 
K5824b smc3-42 trp 
K5828b scc2-4 trp1 
2788 Mata esp1-1 ura3 leu2 can1 lys2 
VG2524-1A Mata PDS5-6MYC pGAL-TRP1:trp1 leu2 ura3 his3 bar1 lys2 
VG2525-2B Mata PDS5-6MYC pGAL-SMT4-TRP1:trp1 leu2 ura3 lys2 his3 bar1 
VG2465-4D Mata PDS5-6MYC smt4::HIS3 leu2 his3 bar1 
VG2463-1D Mata smt4::HIS3 leu2 his3 bar1 
VG2390-37Ac Mata TETGFP-LEU2:leu2 TetOx224-URA3:ura3 trp1 bar1 
VG2416-12Ac pds5-2 
VG2452-7Ac pGAL-TRP1:trp1 
VG2450-7Ac PDS1-3HA-KAN 
VG2456-5Cc pds5-2 PDS1-3HA-KAN 
VG2445-5Bc pds5-2 pGAL-SMT4-TRP1:trp 
VG2446-6Ac pds5-2 pGAL-TRP1:trp1 
VG2452-7Ac pGAL-TRP1:trp1 
VG2485-3Bc pds5-2 pGAL1-SMT4-TRP1:trp1 PDS1-3HA-KAN 
VG2486-14Ac pds5-2 pGAL1-TRP1:trp1 PDS1-3HA-KAN 
VG2259-6B
 
Mata pds5::URA3 trp1 leu2 ura3 + pTH10 (PDS5 CEN LEU2)
 
a

Strains were identical to VG982-6A except for genotypes shown.

b

Strains were identical to K5832 except for genotypes shown (mcd1-73/scc1-73 only in K5832), and were provided by K. Nasmyth (Research Institute of Molecular Pathology, Vienna, Austria).

c

Strains were identical to VG2390-37A except for genotypes shown.

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