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Table II.

High-dosage suppression of the exocyst mutants by SRO7


Mutants

Suppression by 2μ SRO7
sec1-1 — 
sec2-41 — 
*sec3-2 +++ 
*sec3Δ ++ 
sec4-8 — 
*sec5-19 ++ 
*sec6-4 — 
*sec8-9 
sec9-4 — 
*sec10-2 
*sec15-1 ++ 
*exo70-38 +++ 
*exo70Δ ++ 
*exo84-202 ++ 
*exo84Δ
 

 

Mutants

Suppression by 2μ SRO7
sec1-1 — 
sec2-41 — 
*sec3-2 +++ 
*sec3Δ ++ 
sec4-8 — 
*sec5-19 ++ 
*sec6-4 — 
*sec8-9 
sec9-4 — 
*sec10-2 
*sec15-1 ++ 
*exo70-38 +++ 
*exo70Δ ++ 
*exo84-202 ++ 
*exo84Δ
 

 

Growth of post-Golgi secretory mutants transformed with the SRO7 2μ plasmid at the restrictive temperatures.*, The exocyst mutants. Plus signs are used to score the suppression strength of the mutants by 2μ SRO7: ++++, the highest degree of rescuing activity (the same growth rate as the wild type cells); +, the lowest rescuing activity (slight growth at the restrictive temperature).

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