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Table I.

Summary of the kinetic parameters of recombinant C. elegans kinesin-II and sea urchin kinesin-1

Substrate or inhibitor
Recombinant C. elegans kinesin-II
Sea urchin kinesin-1
Mg-ATP Km = 0.28 ± 0.05 mM Vmax = 0.26 ± 0.01 μm/s Km = 0.063 ± 0.034 mM Vmax = 0.56 ± 0.10 μm/s 
Mg-GTP Km = 5.5 mM Vmax = 0.058 μm/s Km = 1.9 ± 0.8 mM Vmax = 0.43 ± 0.08 μm/s 
Mg-ADP Competitive inhibitor Ki = 0.042 mM Competitive inhibitor Ki = 0.156 ± 0.052 mM 
Mg-ATP–γ-Sa Competitive inhibitor Ki = 0.31 mM Competitive inhibitor Ki = 0.014 ± 0.003 mM 
Pi Competitive inhibitor Ki = 4.4 mM Unknown 
Mg-AMPPCP Inhibitor - not a competitive inhibitor Inhibitor - not a competitive inhibitor 
Mg-AMPPNP Inhibitor - not a competitive inhibitor Inhibitor - not a competitive inhibitor 
Substrate or inhibitor
Recombinant C. elegans kinesin-II
Sea urchin kinesin-1
Mg-ATP Km = 0.28 ± 0.05 mM Vmax = 0.26 ± 0.01 μm/s Km = 0.063 ± 0.034 mM Vmax = 0.56 ± 0.10 μm/s 
Mg-GTP Km = 5.5 mM Vmax = 0.058 μm/s Km = 1.9 ± 0.8 mM Vmax = 0.43 ± 0.08 μm/s 
Mg-ADP Competitive inhibitor Ki = 0.042 mM Competitive inhibitor Ki = 0.156 ± 0.052 mM 
Mg-ATP–γ-Sa Competitive inhibitor Ki = 0.31 mM Competitive inhibitor Ki = 0.014 ± 0.003 mM 
Pi Competitive inhibitor Ki = 4.4 mM Unknown 
Mg-AMPPCP Inhibitor - not a competitive inhibitor Inhibitor - not a competitive inhibitor 
Mg-AMPPNP Inhibitor - not a competitive inhibitor Inhibitor - not a competitive inhibitor 
a

Mg-ATP–γ-S can be used as an alternative substrate by recombinant C. elegans kinesin-II. The velocity is 0.0100 ± 0.0015 μm/s at 2 mM Mg-ATP–γ-S.

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