Yeast two-hybrid assay of LF proteins
| DNA-binding plasmid . | DNA-activating plasmid . | β-galactosidase . |
|---|---|---|
| Miller units | ||
| LF2 | LF1 | 12.5 ± 0.1 |
| LF2 | LF3 | 15.8 ± 3.2 |
| LF2 | LF4 | 1.0 ± 0.1 |
| LF2 | SV40 T-antigen | 1.5 ± 0.2 |
| LF4 | LF1 | 0.4 ± 0.1 |
| LF4 | LF3 | 0.4 ± 0.0 |
| LF1 | LF3 | 61.1 ± 3.2 |
| DNA-binding plasmid . | DNA-activating plasmid . | β-galactosidase . |
|---|---|---|
| Miller units | ||
| LF2 | LF1 | 12.5 ± 0.1 |
| LF2 | LF3 | 15.8 ± 3.2 |
| LF2 | LF4 | 1.0 ± 0.1 |
| LF2 | SV40 T-antigen | 1.5 ± 0.2 |
| LF4 | LF1 | 0.4 ± 0.1 |
| LF4 | LF3 | 0.4 ± 0.0 |
| LF1 | LF3 | 61.1 ± 3.2 |
The results were obtained from a representative experiment in which two different yeast transformants were assayed for each combination of proteins. SV40 T-antigen was used as a negative control for interaction.