Involvement of DNA break repair mechanisms in the cellular response to damaged interphase centromeres
| Proteins involved in
DNA break repaira . | Accumulation at centromeres
in ICP0-expressing cells . | Remarks . |
|---|---|---|
| γ-H2AX | No | NA |
| BLM | No | NA |
| Rad51 | No | NA |
| DNA-PKcs | No | Degraded in an ICP0- and proteasomal- dependent mannerb |
| RPA32 | No | NA |
| P-RPA32 | No | NA |
| p53 | No | NA |
| p62 (TFIIH) | No | CB-associated protein (see Table I and Fig. 3) |
| Proteins involved in
DNA break repaira . | Accumulation at centromeres
in ICP0-expressing cells . | Remarks . |
|---|---|---|
| γ-H2AX | No | NA |
| BLM | No | NA |
| Rad51 | No | NA |
| DNA-PKcs | No | Degraded in an ICP0- and proteasomal- dependent mannerb |
| RPA32 | No | NA |
| P-RPA32 | No | NA |
| p53 | No | NA |
| p62 (TFIIH) | No | CB-associated protein (see Table I and Fig. 3) |
γ-H2AX is rapidly phosphorylated on S139 in response to DNA damage. BLM is a helicase from the recQ subfamily that is involved in the cellular response to DNA damage and stalled replication forks. It participates with Rad51 in homologous recombination. DNA-PK plays a central role in the nonhomol ogous end-joining DNA pathway. DNA-PKcs is the catalytic subunit of DNA-PK. Replication protein A (RPA) is a single-stranded DNA-binding protein that is composed of three subunits of 70, 32, and 14 kD. RPA is essential for the recombination and DNA repair pathways. RPA32 becomes hyperphosphorylated on S4 and S8 in response to DNA damage. p62 is a core subunit of the transcriptional/repair factor TFIIH, which is involved in the nucleotide excision repair pathway. NA, not applicable.
Nucleotide excision, base excision, and double-strand breaks.
As shown previously (Parkinson et al., 1999).