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Table I.

Structure of F-actin contractile rings obtained by analyses of equatorially sectioned images


Ring #1
Ring #2
Ring #3
Ring #4
Ring #5
Ring #6
Cell cycle stage early anaphase B early anaphase B late anaphase B late anaphase B cytokinesis cytokinesis 
Number of serial sections 14 20 32 19 33 26 
Number of total filaments in each
     F-actin ring (%) 1,119
 (100) 1,571
 (100) 1,460
 (100) 2,000
 (100) 2,112
 (100) 1,066
 (100) 
Number of clockwise filaments
     (%) 468
 (41.8) 710
 (45.2) 509
 (34.9) 899
 (45) 731
 (34.6) 356
 (33.3) 
Number of counterclockwise
     filaments (%) 581
 (51.9) 645
 (41.1) 676
 (46.3) 873
 (43.7) 886
 (42.0) 427
 (40) 
Number of other filaments
     (%) 70
 (6.3) 216
 (13.7) 275
 (18.8) 228 (11) 495
 (23.4) 283
 (26.5) 
Length of F-actin
     (average ± SD μm) 0.61 ± 0.16 0.59 ± 0.16 0.59 ± 0.15 0.58 ± 0.17 0.45 ± 0.11 0.45 ± 0.13 
Estimated diameter of the ring (μm) 2.31 2.38 2.33 2.54 1.43 1.89 

Ring #1
Ring #2
Ring #3
Ring #4
Ring #5
Ring #6
Cell cycle stage early anaphase B early anaphase B late anaphase B late anaphase B cytokinesis cytokinesis 
Number of serial sections 14 20 32 19 33 26 
Number of total filaments in each
     F-actin ring (%) 1,119
 (100) 1,571
 (100) 1,460
 (100) 2,000
 (100) 2,112
 (100) 1,066
 (100) 
Number of clockwise filaments
     (%) 468
 (41.8) 710
 (45.2) 509
 (34.9) 899
 (45) 731
 (34.6) 356
 (33.3) 
Number of counterclockwise
     filaments (%) 581
 (51.9) 645
 (41.1) 676
 (46.3) 873
 (43.7) 886
 (42.0) 427
 (40) 
Number of other filaments
     (%) 70
 (6.3) 216
 (13.7) 275
 (18.8) 228 (11) 495
 (23.4) 283
 (26.5) 
Length of F-actin
     (average ± SD μm) 0.61 ± 0.16 0.59 ± 0.16 0.59 ± 0.15 0.58 ± 0.17 0.45 ± 0.11 0.45 ± 0.13 
Estimated diameter of the ring (μm) 2.31 2.38 2.33 2.54 1.43 1.89 

The average length of F-actin was obtained for randomly extracted 100 F-actins. Some of these F-actins spanned two serial sections. Diameter of the ring was estimated as follows. First, the area enclosed by a median circle of a ring, which passes through midpoints of the width of the ring was estimated using NIH Image. The diameter was then calculated supposing that the median circle was a complete circle.

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