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Table I.

Quantification of mitotic defects after 48-h PRP4 RNAi in HeLa cells

RNAi
 treatment
Prophase
Prometaphase
Metaphase
Anaphase
Telophase
Lagging chromatids
 during
 anaphase/telophase
 % % % % % % 
Control RNAi 7.8 ± 1.5 28.4 ± 4.6 46.4 ± 3.6 8.8 ± 2.4 7.0 ± 1.4 1.6 ± 1.5 
PRP4 RNAi 9.7 ± 5.1 42.9 ± 7.5 11.9 ± 13.2 6.0 ± 2.7 7.3 ± 6.3 22.2 ± 11.6 
RNAi
 treatment
Prophase
Prometaphase
Metaphase
Anaphase
Telophase
Lagging chromatids
 during
 anaphase/telophase
 % % % % % % 
Control RNAi 7.8 ± 1.5 28.4 ± 4.6 46.4 ± 3.6 8.8 ± 2.4 7.0 ± 1.4 1.6 ± 1.5 
PRP4 RNAi 9.7 ± 5.1 42.9 ± 7.5 11.9 ± 13.2 6.0 ± 2.7 7.3 ± 6.3 22.2 ± 11.6 

Quantification of mitotic defects after 48-h PRP4 RNAi in HeLa cells. Distribution of mitotic cells in the different stages of mitosis: prophase, prometaphase, metaphase, anaphase, and telophase. The values indicate the percentage of cells in each phase ± SD. A supplemental class has been added for aberrant mitosis. PRP4-depleted cells exhibited abnormal anaphase and telophase figures with lagging chromosomes. Note that the mitotic index is two times lower in PRP4-depleted cells.

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