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Table I.

Summary of purified proteins used in biochemical assays (all His tagged)

Construct name
Residues
Extinction coefficient
Drosophila isoforms   
    NTSpir 1–520 25,575a cm−1 
    KIND 1–327 17,452ab cm−1 
    WH2 366–482 15,220b cm−1 
    Capu-FH2 573–1,058 53,760a cm−1 
    Capu-FH1FH2 467–1,058 75,200a cm−1 
Mammalian isoforms   
    Spir-1–KIND 2–271 22,620c cm−1 
    Fmn2-FH2 1,124–1,567 25,445c cm−1 
Construct name
Residues
Extinction coefficient
Drosophila isoforms   
    NTSpir 1–520 25,575a cm−1 
    KIND 1–327 17,452ab cm−1 
    WH2 366–482 15,220b cm−1 
    Capu-FH2 573–1,058 53,760a cm−1 
    Capu-FH1FH2 467–1,058 75,200a cm−1 
Mammalian isoforms   
    Spir-1–KIND 2–271 22,620c cm−1 
    Fmn2-FH2 1,124–1,567 25,445c cm−1 
a

Quantitative SDS-PAGE with Sypro-Red staining.

b

Comparison of absorbance at 280 nM of native protein with protein denatured in 6 M GnHCl.

c

Analytical ultracentrifugation.

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