Expression of the human cofilin plasmid can rescue the phentotypic changes seen in CF KD cells
. | Maximum lengtha
(μm) . | Perimeter
(μm/min) . | Roundednessb
(%) . | Area
(μm2) . |
|---|---|---|---|---|
| Control | 52.16 ± 0.97 | 148.28 ± 3.2 | 73.68 ± 1.32 | 1,285.35 ± 46.71 |
| Cofilin siRNA knockdown | 99.33 ± 6.2 | 257.02 ± 15.13 | 32.87 ± 3.18 | 1,272.26 ± 37.13 |
| Cofilin siRNA knockdown plus WT (ΔH) cofilin | 50.04 ± 2.16 | 137.18 ± 5.13 | 74.80 ± 2.14 | 1,111.87 ± 67.44 |
. | Maximum lengtha
(μm) . | Perimeter
(μm/min) . | Roundednessb
(%) . | Area
(μm2) . |
|---|---|---|---|---|
| Control | 52.16 ± 0.97 | 148.28 ± 3.2 | 73.68 ± 1.32 | 1,285.35 ± 46.71 |
| Cofilin siRNA knockdown | 99.33 ± 6.2 | 257.02 ± 15.13 | 32.87 ± 3.18 | 1,272.26 ± 37.13 |
| Cofilin siRNA knockdown plus WT (ΔH) cofilin | 50.04 ± 2.16 | 137.18 ± 5.13 | 74.80 ± 2.14 | 1,111.87 ± 67.44 |
DIAS analysis was carried to quantify shape parameters for control, CF KD cells, and CF KD cells + WT(ΔH) cofilin plasmid.
Maximum length is the distance between the farthest points on the cell boundary.
Roundedness is a measure of how efficiently a given amount of perimeter encloses an area (a circle has 100% roundedness; a straight line has 0%). Number of cells scored: control (35), CF KD (31) and cofilin CF KD + WT (ΔH) (19). Numbers are significantly different (P < 0.0001) between control and CF KD groups, and between CF KD and CF KD+WT(ΔH) cofilin groups, for all parameters except for the area measurement.