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Table I.

Quantitation of MT dynamic behavior in PP4c^−/− MEFs

Used MEF transfection	CKO/CKO	PP4c^−/−	PP4^CKO/CKO  control RNAi	PP4^CKO/CKO  p60 RNAi	PP4^CKO/CKO  p60 MT	PP4c^−/−  control RNAi	PP4c^−/−  p60 RNAi	PP4c^−/−  p60 MT
Growth rate (mm/min)	10.31 ± 0.65	13.62 ± 0.88	11.74 ± 0.45	14.32 ± 0.57	13.89 ± 0.33	13.20 ± 0.47	13.63 ± 0.34	13.50 ± 0.48
Shrink rate (mm/min)	17.93 ± 1.11	20.29 ± 1.29	18.54 ± 1.63	17.82 ± 0.53	17.14 ± 0.92	19.83 ± 1.15	18.89 ± 0.65	18.23 ± 1.90
Percent time spent
Growth	30.7	17.5	25.5	56.3	41.2	13.4	34.3	30.2
Shrink	20.1	16.2	18.2	22.2	21.6	13.4	21.7	20.1
Pause	49.2	66.3	56.3	24.2	37.2	73.2	44	49.7
Catastrophe frequency  (min⁻¹)	0.98 ± 0.09	1.60 ± 1.10	1.03 ± 0.12	1.37 ± 0.18	1.44 ± 0.09	1.70 ± 0.07	1.05 ± 0.07	1.05 ± 0.10
Rescue frequency  (min⁻¹)	1.78 ± 0.10	1.65 ± 0.08	1.69 ± 0.12	1.93 ± 0.14	1.94 ± 0.07	1.59 ± 0.10	1.91 ± 0.08	2.04 ± 0.29
No. of MTs	146	153	160	122	108	102	140	138
No. of cells	11	14	14	13	10	8	9	12

Used MEF transfection	CKO/CKO	PP4c^−/−	PP4^CKO/CKO  control RNAi	PP4^CKO/CKO  p60 RNAi	PP4^CKO/CKO  p60 MT	PP4c^−/−  control RNAi	PP4c^−/−  p60 RNAi	PP4c^−/−  p60 MT
Growth rate (mm/min)	10.31 ± 0.65	13.62 ± 0.88	11.74 ± 0.45	14.32 ± 0.57	13.89 ± 0.33	13.20 ± 0.47	13.63 ± 0.34	13.50 ± 0.48
Shrink rate (mm/min)	17.93 ± 1.11	20.29 ± 1.29	18.54 ± 1.63	17.82 ± 0.53	17.14 ± 0.92	19.83 ± 1.15	18.89 ± 0.65	18.23 ± 1.90
Percent time spent
Growth	30.7	17.5	25.5	56.3	41.2	13.4	34.3	30.2
Shrink	20.1	16.2	18.2	22.2	21.6	13.4	21.7	20.1
Pause	49.2	66.3	56.3	24.2	37.2	73.2	44	49.7
Catastrophe frequency  (min⁻¹)	0.98 ± 0.09	1.60 ± 1.10	1.03 ± 0.12	1.37 ± 0.18	1.44 ± 0.09	1.70 ± 0.07	1.05 ± 0.07	1.05 ± 0.10
Rescue frequency  (min⁻¹)	1.78 ± 0.10	1.65 ± 0.08	1.69 ± 0.12	1.93 ± 0.14	1.94 ± 0.07	1.59 ± 0.10	1.91 ± 0.08	2.04 ± 0.29
No. of MTs	146	153	160	122	108	102	140	138
No. of cells	11	14	14	13	10	8	9	12

p60 RNAi, siRNA against katanin p60; p60 MT, katanin p60 mutated plasmid. We selected type A PP4c2/2 MEF for examination.