Summary of HLB assembly and histone pre-mRNA processing requirements
| Gene transcript targeted by RNAi | Reduced MPM-2 index | Reduced Mxc index | Reduced FLASH index | Reduced Mute index | Scored positive with histone misprocessing reporter |
| mxc | ++ | ++ | ++ | ++ | Yesa |
| FLASH | ++ | + | ++ | + | Yes |
| Int6 | ++ | − | − | − | No |
| CSN3 | ++ | − | − | − | No |
| CSN4 | ++ | − | − | − | No |
| CSN5 | ++ | − | − | − | No |
| CG4849 | ++ | − | − | − | No |
| CG9769 | ++ | − | − | ++ | No |
| CG7597 | + | − | − | ++ | No |
| Tbp-1 | + | − | − | − | No |
| HLH106 | + | − | − | − | No |
| RpII18 | + | − | − | − | No |
| eIF-3p66 | + | − | − | − | No |
| alien | + | − | − | − | No |
| CG17841 | + | − | − | − | No |
| foi | + | − | − | − | No |
| Fer1HCH | + | − | − | − | No |
| CG11985 | + | − | − | − | No |
| CG18591/SmE | + | − | + | − | Yesa |
| MBD-R2 | + | − | ++ | + | Yes |
| CG18787/18789 | + | − | − | − | No |
| CG9121 | + | − | − | − | No |
| CG5844 | + | − | − | − | No |
| CG5147 | + | − | − | − | No |
| mfrn | + | − | − | + | No |
| CG14111 | + | − | − | − | No |
| Mute | − | − | − | ++ | No |
| Nnp-1 | − | − | − | + | No |
| raptor | − | − | − | + | No |
| MCRS1 | − | − | − | − | Yes |
| CG31111 | − | − | − | − | Yesa |
| CG8142 | − | − | − | − | Yesa |
| DMAP1 | − | − | − | − | Yesa |
| Dgt1 | − | − | − | − | Yesa |
| CG9772/Skp2 | − | − | − | − | Yesa |
| Gene transcript targeted by RNAi | Reduced MPM-2 index | Reduced Mxc index | Reduced FLASH index | Reduced Mute index | Scored positive with histone misprocessing reporter |
| mxc | ++ | ++ | ++ | ++ | Yesa |
| FLASH | ++ | + | ++ | + | Yes |
| Int6 | ++ | − | − | − | No |
| CSN3 | ++ | − | − | − | No |
| CSN4 | ++ | − | − | − | No |
| CSN5 | ++ | − | − | − | No |
| CG4849 | ++ | − | − | − | No |
| CG9769 | ++ | − | − | ++ | No |
| CG7597 | + | − | − | ++ | No |
| Tbp-1 | + | − | − | − | No |
| HLH106 | + | − | − | − | No |
| RpII18 | + | − | − | − | No |
| eIF-3p66 | + | − | − | − | No |
| alien | + | − | − | − | No |
| CG17841 | + | − | − | − | No |
| foi | + | − | − | − | No |
| Fer1HCH | + | − | − | − | No |
| CG11985 | + | − | − | − | No |
| CG18591/SmE | + | − | + | − | Yesa |
| MBD-R2 | + | − | ++ | + | Yes |
| CG18787/18789 | + | − | − | − | No |
| CG9121 | + | − | − | − | No |
| CG5844 | + | − | − | − | No |
| CG5147 | + | − | − | − | No |
| mfrn | + | − | − | + | No |
| CG14111 | + | − | − | − | No |
| Mute | − | − | − | ++ | No |
| Nnp-1 | − | − | − | + | No |
| raptor | − | − | − | + | No |
| MCRS1 | − | − | − | − | Yes |
| CG31111 | − | − | − | − | Yesa |
| CG8142 | − | − | − | − | Yesa |
| DMAP1 | − | − | − | − | Yesa |
| Dgt1 | − | − | − | − | Yesa |
| CG9772/Skp2 | − | − | − | − | Yesa |
The table lists those genes of the 95 selected for validation from the primary screen that scored in at least one of the five secondary assays: anti-MPM-2, -Mxc, -FLASH, or -Mute HLB labeling and activation of the histone pre-mRNA misprocessing reporter. A score of a double plus sign, a single plus sign, or a negative sign was assigned when the spot index fell ≥1.5 standard deviations from the control mean (i.e., −1.5 z score) in each, at least one, or no validation replicate, respectively (also see Table S2). Note that Mxc, FLASH, and Mute all strongly reduced their own index, indicating successful RNAi-mediated depletion. Although the eight genes listed at the bottom of the table did not produce a −1.5 or less z score in any of the MPM-2 validation replicates, they were included because they either activated the misprocessing reporter or affected the Mute index.
Newly identified genes involved in histone pre-mRNA processing.