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Table 1.

Quantification of the nuclear migration defect

Strain Dividing cells with the nucleus only in the mother cell Number of dividing cells 
 %  
NUP159-HA 28 ± 4 260 
nup159K897R-HA 46 ± 0** 198 
DYN2 21 ± 14 341 
dyn2Δ 44 ± 6*a 388 
NUP159 0.54 ± 0.47 472 at 23°C and 557 at 37°C 
nup159-1 1.64 ± 0.27* 440 at 23°C and 375 at 37°C 
Strain Dividing cells with the nucleus only in the mother cell Number of dividing cells 
 %  
NUP159-HA 28 ± 4 260 
nup159K897R-HA 46 ± 0** 198 
DYN2 21 ± 14 341 
dyn2Δ 44 ± 6*a 388 
NUP159 0.54 ± 0.47 472 at 23°C and 557 at 37°C 
nup159-1 1.64 ± 0.27* 440 at 23°C and 375 at 37°C 

Asynchronous population of ethanol-fixed cells of the indicated phenotype were stained with Hoechst and analyzed by fluorescence microscopy for the position of the nucleus. Cells whose buds size reached the dividing size (dividing cells) were scored for the presence of nucleus only in the mother cell. For each strain, the number of divided cells counted is indicated in the right column. One asterisk corresponds to p-values between 0.01 and 0.05, and two asterisks correspond to p-values between 0.001 and 0.01.

a

Value at 37°C normalized to value at 23°C.

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