Strains used in this study
| Strain | Genotype |
| MT670 | MATa ade2 his3 leu2 trp1 ura3 cdc34-2 |
| MT670 NUP159-HA | MT670 NUP159-3HA::hphNT1 |
| W303 NUPX-HA | MATα nupx-3HA::HIS3MX6 |
| W303Δbar1 | MATa bar1::URA3 |
| W303Δbar1 Nup159-HA | MATa bar1::URA3NUP159-3HA::hphNT1 |
| W303Δbar1Δnup159 Nup159-HA | MATa bar1::URA3 nup159::hphNT1 p415ADH Nup159-HA |
| W303Δbar1Δnup159nup159K897R-HA | MATa bar1::URA3 nup159::hphNT1 p415ADH nup159K897R-HA |
| W303Δbar1Δnup159 DYN2-GFP Nup159-HA | MATa bar1::URA3 nup159::hphNT1 Nup159-HA DYN2- GFP::KanMX4 |
| W303Δbar1Δnup159DYN2-GFP,nup159K897R-HA | MATa bar1::URA3 nup159::hphNT1 nup159K897R-HA DYN2-GFP::KanMX4 |
| BY4741 NUP60-GFP NUP159-mCherry | BY4741 nup60-GFP::HIS3MX6 nup159-mCherry::KanMX |
| BY4741 NUP60-GFP NUP159K897R-mCherry | BY4741nup60-GFP::HIS3MX6nup159K897R-mCherry::KanMX |
| W303Δkar9Δnup159 Nup159-HA | MAT? kar9::KanMX4 nup159::hphNT1 p415ADH Nup159-HA |
| W303Δkar9Δnup159 nup159K897R-HA | MAT? kar9::KanMX4 nup159::hphNT1 p415ADH nup159K897R-HA |
| BY4741 DYN2-GFP | BY4741 DYN2-GFP::HIS3MX6 |
| BY4741 DYN2-GFP grr1Δ | BY4741 DYN2-GFP::HIS3MX6 grr1Δ::KanMX |
| W303Δnup159 Nup159-HA Cdc10-mCherry | MATα CDC10-mCherry::KanMX4nup159::hphNT1 p415ADH Nup159-HA |
| W303Δnup159 nup159K897R-HA Cdc10-mCherry | MATα CDC10-mCherry::KanMX4nup159::hphNT1 p415ADH nup159K897R-HA |
| MT670 Nup159-mCherry | MATa ade2his3leu2trp1ura3cdc34-2NUP159-mCherry::KanMX4 |
| Strain | Genotype |
| MT670 | MATa ade2 his3 leu2 trp1 ura3 cdc34-2 |
| MT670 NUP159-HA | MT670 NUP159-3HA::hphNT1 |
| W303 NUPX-HA | MATα nupx-3HA::HIS3MX6 |
| W303Δbar1 | MATa bar1::URA3 |
| W303Δbar1 Nup159-HA | MATa bar1::URA3NUP159-3HA::hphNT1 |
| W303Δbar1Δnup159 Nup159-HA | MATa bar1::URA3 nup159::hphNT1 p415ADH Nup159-HA |
| W303Δbar1Δnup159nup159K897R-HA | MATa bar1::URA3 nup159::hphNT1 p415ADH nup159K897R-HA |
| W303Δbar1Δnup159 DYN2-GFP Nup159-HA | MATa bar1::URA3 nup159::hphNT1 Nup159-HA DYN2- GFP::KanMX4 |
| W303Δbar1Δnup159DYN2-GFP,nup159K897R-HA | MATa bar1::URA3 nup159::hphNT1 nup159K897R-HA DYN2-GFP::KanMX4 |
| BY4741 NUP60-GFP NUP159-mCherry | BY4741 nup60-GFP::HIS3MX6 nup159-mCherry::KanMX |
| BY4741 NUP60-GFP NUP159K897R-mCherry | BY4741nup60-GFP::HIS3MX6nup159K897R-mCherry::KanMX |
| W303Δkar9Δnup159 Nup159-HA | MAT? kar9::KanMX4 nup159::hphNT1 p415ADH Nup159-HA |
| W303Δkar9Δnup159 nup159K897R-HA | MAT? kar9::KanMX4 nup159::hphNT1 p415ADH nup159K897R-HA |
| BY4741 DYN2-GFP | BY4741 DYN2-GFP::HIS3MX6 |
| BY4741 DYN2-GFP grr1Δ | BY4741 DYN2-GFP::HIS3MX6 grr1Δ::KanMX |
| W303Δnup159 Nup159-HA Cdc10-mCherry | MATα CDC10-mCherry::KanMX4nup159::hphNT1 p415ADH Nup159-HA |
| W303Δnup159 nup159K897R-HA Cdc10-mCherry | MATα CDC10-mCherry::KanMX4nup159::hphNT1 p415ADH nup159K897R-HA |
| MT670 Nup159-mCherry | MATa ade2his3leu2trp1ura3cdc34-2NUP159-mCherry::KanMX4 |