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Table 2.

Strains used in this study

Strain Genotype 
MT670 MATa ade2 his3 leu2 trp1 ura3 cdc34-2 
MT670 NUP159-HA MT670 NUP159-3HA::hphNT1 
W303 NUPX-HA MATα nupx-3HA::HIS3MX6 
W303Δbar1 MATa bar1::URA3 
W303Δbar1 Nup159-HA MATa bar1::URA3NUP159-3HA::hphNT1 
W303Δbar1Δnup159 Nup159-HA MATa bar1::URA3 nup159::hphNT1 p415ADH Nup159-HA 
W303Δbar1Δnup159nup159K897R-HA MATa bar1::URA3 nup159::hphNT1 p415ADH nup159K897R-HA 
W303Δbar1Δnup159 DYN2-GFP Nup159-HA MATa bar1::URA3 nup159::hphNT1 Nup159-HA DYN2- GFP::KanMX4 
W303Δbar1Δnup159DYN2-GFP,nup159K897R-HA MATa bar1::URA3 nup159::hphNT1 nup159K897R-HA DYN2-GFP::KanMX4 
BY4741 NUP60-GFP NUP159-mCherry BY4741 nup60-GFP::HIS3MX6 nup159-mCherry::KanMX 
BY4741 NUP60-GFP NUP159K897R-mCherry BY4741nup60-GFP::HIS3MX6nup159K897R-mCherry::KanMX 
W303Δkar9Δnup159 Nup159-HA MAT? kar9::KanMX4 nup159::hphNT1 p415ADH Nup159-HA 
W303Δkar9Δnup159 nup159K897R-HA MAT? kar9::KanMX4 nup159::hphNT1 p415ADH nup159K897R-HA 
BY4741 DYN2-GFP BY4741 DYN2-GFP::HIS3MX6 
BY4741 DYN2-GFP grr1Δ BY4741 DYN2-GFP::HIS3MX6 grr1Δ::KanMX 
W303Δnup159 Nup159-HA Cdc10-mCherry MATα CDC10-mCherry::KanMX4nup159::hphNT1 p415ADH Nup159-HA 
W303Δnup159 nup159K897R-HA Cdc10-mCherry MATα CDC10-mCherry::KanMX4nup159::hphNT1 p415ADH nup159K897R-HA 
MT670 Nup159-mCherry MATa ade2his3leu2trp1ura3cdc34-2NUP159-mCherry::KanMX4 
Strain Genotype 
MT670 MATa ade2 his3 leu2 trp1 ura3 cdc34-2 
MT670 NUP159-HA MT670 NUP159-3HA::hphNT1 
W303 NUPX-HA MATα nupx-3HA::HIS3MX6 
W303Δbar1 MATa bar1::URA3 
W303Δbar1 Nup159-HA MATa bar1::URA3NUP159-3HA::hphNT1 
W303Δbar1Δnup159 Nup159-HA MATa bar1::URA3 nup159::hphNT1 p415ADH Nup159-HA 
W303Δbar1Δnup159nup159K897R-HA MATa bar1::URA3 nup159::hphNT1 p415ADH nup159K897R-HA 
W303Δbar1Δnup159 DYN2-GFP Nup159-HA MATa bar1::URA3 nup159::hphNT1 Nup159-HA DYN2- GFP::KanMX4 
W303Δbar1Δnup159DYN2-GFP,nup159K897R-HA MATa bar1::URA3 nup159::hphNT1 nup159K897R-HA DYN2-GFP::KanMX4 
BY4741 NUP60-GFP NUP159-mCherry BY4741 nup60-GFP::HIS3MX6 nup159-mCherry::KanMX 
BY4741 NUP60-GFP NUP159K897R-mCherry BY4741nup60-GFP::HIS3MX6nup159K897R-mCherry::KanMX 
W303Δkar9Δnup159 Nup159-HA MAT? kar9::KanMX4 nup159::hphNT1 p415ADH Nup159-HA 
W303Δkar9Δnup159 nup159K897R-HA MAT? kar9::KanMX4 nup159::hphNT1 p415ADH nup159K897R-HA 
BY4741 DYN2-GFP BY4741 DYN2-GFP::HIS3MX6 
BY4741 DYN2-GFP grr1Δ BY4741 DYN2-GFP::HIS3MX6 grr1Δ::KanMX 
W303Δnup159 Nup159-HA Cdc10-mCherry MATα CDC10-mCherry::KanMX4nup159::hphNT1 p415ADH Nup159-HA 
W303Δnup159 nup159K897R-HA Cdc10-mCherry MATα CDC10-mCherry::KanMX4nup159::hphNT1 p415ADH nup159K897R-HA 
MT670 Nup159-mCherry MATa ade2his3leu2trp1ura3cdc34-2NUP159-mCherry::KanMX4 
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