Table 1.

Peptide sequences of the 85-kD axonemal protein copurified with RSP31–178-HAHis

Scan no. Sequence 
LTQLAELAR 
VDPYVVAYVTSLEDAR 
AFLSDPAAVLAGVAAAVAR 
VYGFSSNADMR 
GALQSLVPDVSLK 
AALESVFPVSAVSR 
AGAVSSEAVAAIGGYVAGLVGDAR 
FVTLAEGER 
Scan no. Sequence 
LTQLAELAR 
VDPYVVAYVTSLEDAR 
AFLSDPAAVLAGVAAAVAR 
VYGFSSNADMR 
GALQSLVPDVSLK 
AALESVFPVSAVSR 
AGAVSSEAVAAIGGYVAGLVGDAR 
FVTLAEGER 

The protein band of the Ni-NTA pull-down sample in a silver-stained gel was excised, and the peptide sequences were revealed by liquid chromatography–tandem mass spectrometry. All correspond to flagella-associated protein FAP206.

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