Peptide sequences of the 85-kD axonemal protein copurified with RSP31–178-HAHis
Scan no.
Sequence
7
LTQLAELAR
6
VDPYVVAYVTSLEDAR
5
AFLSDPAAVLAGVAAAVAR
4
VYGFSSNADMR
3
GALQSLVPDVSLK
3
AALESVFPVSAVSR
1
AGAVSSEAVAAIGGYVAGLVGDAR
1
FVTLAEGER
Scan no.
Sequence
7
LTQLAELAR
6
VDPYVVAYVTSLEDAR
5
AFLSDPAAVLAGVAAAVAR
4
VYGFSSNADMR
3
GALQSLVPDVSLK
3
AALESVFPVSAVSR
1
AGAVSSEAVAAIGGYVAGLVGDAR
1
FVTLAEGER
The protein band of the Ni-NTA pull-down sample in a silver-stained gel was excised, and the peptide sequences were revealed by liquid chromatography–tandem mass spectrometry. All correspond to flagella-associated protein FAP206.