Rockefeller University Press

Table 2.

Corralled diffusion fitting parameters: high-speed (200-fps) tracking

Cilium	Cilium length	D_m1^{^a}	D_m2^{^b}	D_cor^{^c}	P_bc^{^d}	l_cor^{^e}	T_r^{^f}
	μm	μm²/s	μm²/s	μm²/s		Μm	s
R9	3.8	2.2	0.24	4.6	0.0024	0.147	0.61
R10	2.3	0.83	0.02	2.83	0.0008	0.274	1.21
R11	5.3	3.4	0.16	6.4	0.0012	0.367	1.20
R12	2.8	4.7	0.34	8.4	0.0032	0.274	0.48
R13	2.9	2.6	0.16	5.2	0.0018	0.315	0.85
R14	2.3	5.1	0.27	8.5	0.0016	0.367	1.39
R14	3.8	3.2	0.12	4.3	0.0014	0.421	1.41
R15	2.1	0.78	0.18	3.2	0.0083	0.130	0.13
R16	4.5	1.3	0.11	3.3	0.0033	0.183	0.39
R17	3.0	1.7	0.25	4.8	0.0056	0.222	0.25
R18	5.1	2.0	0.14	4.8	0.0018	0.239	0.82
S12	7.3	2.8	0.18	4.3	0.0017	0.245	0.94
S13	5.9	7.5	0.11	7.5	0.0033	0.431	0.67
S14	3.3	2.7	0.25	7.7	0.0040	0.268	0.29
S15	3.8	5	0.14	8	0.0031	0.395	0.50
S16	7.1	1.4	0.10	7	0.0022	0.205	0.57
S17	2.3	1.1	0.10	4.2	0.0026	0.173	0.46
S18	3.3	3.2	0.11	3.2	0.0026	0.329	0.53
Mean	3.9	2.9	0.235	5.46	0.0028	0.274	0.71
SEM	0.37	0.4	0.046	0.45	0.0004	0.020	0.09

Cilium	Cilium length	D_m1^{^a}	D_m2^{^b}	D_cor^{^c}	P_bc^{^d}	l_cor^{^e}	T_r^{^f}
	μm	μm²/s	μm²/s	μm²/s		Μm	s
R9	3.8	2.2	0.24	4.6	0.0024	0.147	0.61
R10	2.3	0.83	0.02	2.83	0.0008	0.274	1.21
R11	5.3	3.4	0.16	6.4	0.0012	0.367	1.20
R12	2.8	4.7	0.34	8.4	0.0032	0.274	0.48
R13	2.9	2.6	0.16	5.2	0.0018	0.315	0.85
R14	2.3	5.1	0.27	8.5	0.0016	0.367	1.39
R14	3.8	3.2	0.12	4.3	0.0014	0.421	1.41
R15	2.1	0.78	0.18	3.2	0.0083	0.130	0.13
R16	4.5	1.3	0.11	3.3	0.0033	0.183	0.39
R17	3.0	1.7	0.25	4.8	0.0056	0.222	0.25
R18	5.1	2.0	0.14	4.8	0.0018	0.239	0.82
S12	7.3	2.8	0.18	4.3	0.0017	0.245	0.94
S13	5.9	7.5	0.11	7.5	0.0033	0.431	0.67
S14	3.3	2.7	0.25	7.7	0.0040	0.268	0.29
S15	3.8	5	0.14	8	0.0031	0.395	0.50
S16	7.1	1.4	0.10	7	0.0022	0.205	0.57
S17	2.3	1.1	0.10	4.2	0.0026	0.173	0.46
S18	3.3	3.2	0.11	3.2	0.0026	0.329	0.53
Mean	3.9	2.9	0.235	5.46	0.0028	0.274	0.71
SEM	0.37	0.4	0.046	0.45	0.0004	0.020	0.09

GPCR membrane diffusion coefficient computed from slope of first two MSD(τ) points (first phase).

GPCR membrane diffusion coefficient computed from slope of the second phase of the MSD(τ) plot.

GPCR membrane diffusion coefficient found by fitting MSD(τ) with corralled random walk simulation. This represents the D_m that is least affected by corral confinement.

Probability of a GPCR crossing a corral boundary upon encountering the boundary, found by fitting MSD(τ) with corralled random walk simulation.

Mean length of corral in the axial direction, computed from y-intercept of the linear regression of the second phase of the MSD(τ) plot.

Mean residence time for a GPCR within a corral, found by fitting MSD(τ) with corralled random walk simulation.

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