Rockefeller University Press

Table IV.

Survival advantage of IgG1⁺ cells over IgM⁺ competitor B cells

		IgG1⁺/IgM⁺ ratio
		BM			Spleen
Genotype	No.	Immature	Mature/  recirculating	T1	T2/T3	All  transitional	Mature
		AA4.1⁺  B220⁺	AA4.1⁻  B220⁺	AA4.1⁺CD23⁻  CD19⁺	AA4.1⁺CD23⁺  CD19⁺	AA4.1⁺  CD19⁺	AA4.1⁻  CD19⁺
IgH^γ1μ/+ Igα^Δc/+	n = 5	0.06 ± 0.01	0.16 ± 0.02	0.058 ± 0.012	0.106 ± 0.007	0.08 ± 0.01	0.16 ± 0.02
IgH^γ1μ/+ Igα^Δc1/Δc1	n = 5	0.83 ± 0.22	2.24 ± 0.71	ND	ND	2.09 ± 0.29	2.68 ± 0.3

		IgG1⁺/IgM⁺ ratio
		BM			Spleen
Genotype	No.	Immature	Mature/  recirculating	T1	T2/T3	All  transitional	Mature
		AA4.1⁺  B220⁺	AA4.1⁻  B220⁺	AA4.1⁺CD23⁻  CD19⁺	AA4.1⁺CD23⁺  CD19⁺	AA4.1⁺  CD19⁺	AA4.1⁻  CD19⁺
IgH^γ1μ/+ Igα^Δc/+	n = 5	0.06 ± 0.01	0.16 ± 0.02	0.058 ± 0.012	0.106 ± 0.007	0.08 ± 0.01	0.16 ± 0.02
IgH^γ1μ/+ Igα^Δc1/Δc1	n = 5	0.83 ± 0.22	2.24 ± 0.71	ND	ND	2.09 ± 0.29	2.68 ± 0.3

In heterozygous IgH^γ1μ/+ Igα^Δc+/+ and IgH^γ1μ/+ Igα^Δc1/Δc1 mice, the IgG1⁺/IgM⁺ B cell ratio increases with the maturational status. Note the strong increase of the fraction of IgG1⁺ B cell presence within the mature compartment. BM and splenic cells were surface stained for IgG1/IgM, AA4.1, CD23, and a B cell–specific marker (B220 or CD19) and analyzed by flow cytometry.

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