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Table 1.

Strain list

Strain	Genotype	Source
NY605	MATa ura3-52 leu2-3,112	Novick laboratory^{^a}
RCY1507	MATα ura3-52 leu2-3,112 his3Δ200 SEC4ΔHIS3 [YCP50 SEC4]	Calero et al., 2003
CUY318	MATa ade1 ade2 lys2 tyr1 gal1 his7 cdc5^ts	Huffaker laboratory^{^b}
RCY3343	MATa ura3-52 leu2-3,112 his3Δ0	Collins laboratory^{^c}
RCY3350	MATa ura3Δ0 leu2-3,112 cdc55ΔKAN^R	Heger et al., 2011
RCY5066	MATa ura3-52 leu2-3,112 his3Δ0 BAR1ΔHIS3	This study
RCY5009	MATα ura3-52 leu2-3,112 his3Δ200 SEC4ΔHIS3 [pRS315 MBP-SEC4]	This study
RCY5010	MATα ura3-52 leu2-3,112 his3Δ200 SEC4ΔHIS3 [pRS315 MBP-SEC4 S8,10,11,201,204A]	This study
RCY5085A	MATa ura3-52 leu2-3,112 his3Δ0 sch9ΔHIS3	This study
RCY5090	MATα his3Δ0 leu2Δ0 ura3Δ0 env7ΔKAN^R	Resgen^{^d}
RCY5091	MATα his3Δ0 leu2Δ0 ura3Δ0 sky1ΔKAN^R	Resgen
RCY5092	MATα his3Δ0 leu2Δ0 ura3Δ0 pkh3ΔKAN^R	Resgen
RCY5081	MATα his3Δ0 leu2Δ0 ura3Δ0 sak1ΔKAN^R	Resgen
RCY5093	MATα his3Δ0 leu2Δ0 ura3Δ0 slt2ΔKAN^R	Resgen
RCY5094	MATα his3Δ0 leu2Δ0 ura3Δ0 psk2ΔKAN^R	Resgen
RCY5095	MATα his3Δ0 leu2Δ0 ura3Δ0 ste11ΔKAN^R	Resgen
RCY5096	MATα his3Δ0 leu2Δ0 ura3Δ0 vps15ΔKAN^R	Resgen
RCY4607	MATα his3Δ0 leu2Δ0 ura3Δ0 kcc4KAN^R	Resgen
RCY5097	MATα his3Δ0 leu2Δ0 ura3Δ0 bub1ΔKAN^R	Resgen
RCY5130	MATa ura3-52 leu2-3,112 his3Δ200 hof1ΔKAN^R SEC4ΔHIS3 [YCP50 SEC4]	This study
RCY5126	MATa ura3-52 ade2-101 his3Δ200 myo1ΔLEU2 SEC4ΔHIS3 [YCP50 SEC4]	This study
RCY5120	MATα ura3-52 leu2-3,112 his3Δ200 SEC4ΔHIS3 [pRS315 SEC4 S2, 8, 10, 11, 201, 204A; T6A]	This study

Strain	Genotype	Source
NY605	MATa ura3-52 leu2-3,112	Novick laboratory^{^a}
RCY1507	MATα ura3-52 leu2-3,112 his3Δ200 SEC4ΔHIS3 [YCP50 SEC4]	Calero et al., 2003
CUY318	MATa ade1 ade2 lys2 tyr1 gal1 his7 cdc5^ts	Huffaker laboratory^{^b}
RCY3343	MATa ura3-52 leu2-3,112 his3Δ0	Collins laboratory^{^c}
RCY3350	MATa ura3Δ0 leu2-3,112 cdc55ΔKAN^R	Heger et al., 2011
RCY5066	MATa ura3-52 leu2-3,112 his3Δ0 BAR1ΔHIS3	This study
RCY5009	MATα ura3-52 leu2-3,112 his3Δ200 SEC4ΔHIS3 [pRS315 MBP-SEC4]	This study
RCY5010	MATα ura3-52 leu2-3,112 his3Δ200 SEC4ΔHIS3 [pRS315 MBP-SEC4 S8,10,11,201,204A]	This study
RCY5085A	MATa ura3-52 leu2-3,112 his3Δ0 sch9ΔHIS3	This study
RCY5090	MATα his3Δ0 leu2Δ0 ura3Δ0 env7ΔKAN^R	Resgen^{^d}
RCY5091	MATα his3Δ0 leu2Δ0 ura3Δ0 sky1ΔKAN^R	Resgen
RCY5092	MATα his3Δ0 leu2Δ0 ura3Δ0 pkh3ΔKAN^R	Resgen
RCY5081	MATα his3Δ0 leu2Δ0 ura3Δ0 sak1ΔKAN^R	Resgen
RCY5093	MATα his3Δ0 leu2Δ0 ura3Δ0 slt2ΔKAN^R	Resgen
RCY5094	MATα his3Δ0 leu2Δ0 ura3Δ0 psk2ΔKAN^R	Resgen
RCY5095	MATα his3Δ0 leu2Δ0 ura3Δ0 ste11ΔKAN^R	Resgen
RCY5096	MATα his3Δ0 leu2Δ0 ura3Δ0 vps15ΔKAN^R	Resgen
RCY4607	MATα his3Δ0 leu2Δ0 ura3Δ0 kcc4KAN^R	Resgen
RCY5097	MATα his3Δ0 leu2Δ0 ura3Δ0 bub1ΔKAN^R	Resgen
RCY5130	MATa ura3-52 leu2-3,112 his3Δ200 hof1ΔKAN^R SEC4ΔHIS3 [YCP50 SEC4]	This study
RCY5126	MATa ura3-52 ade2-101 his3Δ200 myo1ΔLEU2 SEC4ΔHIS3 [YCP50 SEC4]	This study
RCY5120	MATα ura3-52 leu2-3,112 his3Δ200 SEC4ΔHIS3 [pRS315 SEC4 S2, 8, 10, 11, 201, 204A; T6A]	This study

Novick Laboratory, University of California, San Diego, San Diego, CA.

Huffaker Laboratory, Cornell University, Ithaca, NY.

Collins Laboratory, Cornell University, Ithaca, NY.

Resgen haploid delete collection: http://www-sequence.stanford.edu/group/yeast_deletion_project/.

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